Detailed information of TA system TA230674

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 4154612..4155207
• Replicon: chromosome
• Accession: NZ_CP090402
• Organism: Shigella sp. PIB

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: V0SXT4
• Locus tag: LXH19_RS19650
• Protein ID: WP_000239581.1
• Coordinates: 4154612..4154962 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: L4JJX7
• Locus tag: LXH19_RS19655
• Protein ID: WP_001223213.1
• Coordinates: 4154956..4155207 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LXH19_RS19630 (4150066)	4150066..4151088	-	1023	WP_001468884.1	ABC transporter permease	-
LXH19_RS19635 (4151102)	4151102..4152604	-	1503	WP_001024741.1	sugar ABC transporter ATP-binding protein	-
LXH19_RS19640 (4152737)	4152737..4153693	-	957	WP_000265935.1	galactofuranose ABC transporter substrate-binding protein YtfQ	-
LXH19_RS19645 (4154003)	4154003..4154533	+	531	WP_000055075.1	inorganic diphosphatase	-
LXH19_RS19650 (4154612)	4154612..4154962	-	351	WP_000239581.1	endoribonuclease toxin ChpB	Toxin
LXH19_RS19655 (4154956)	4154956..4155207	-	252	WP_001223213.1	type II toxin-antitoxin system antitoxin ChpS	Antitoxin
LXH19_RS19660 (4155419)	4155419..4155760	-	342	WP_001219160.1	gamma-glutamylcyclotransferase	-
LXH19_RS19665 (4155763)	4155763..4159542	-	3780	WP_000060909.1	autotransporter assembly complex protein TamB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-114)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12453.41 Da
• Isoelectric Point: 6.2206

>T230674 WP_000239581.1 NZ_CP090402:c4154962-4154612 [Shigella sp. PIB]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAAGEVVEEALLRLQAVVE

Nucleotide

• Length: 351 bp

>T230674 NZ_CP125310:564588-564872 [Citrobacter freundii]
ATGACTTATGAACTGGAATTCGACCCGAGAGCCTTAAAAGAGTGGCATAAACTGGGTGATACGGTGAAGGCTCAGTTTAA
GAAGAAGTTGGCCGACGTGTTGTTGAACCCCAGAATTGACTCTGCCCGTTTAAACGGTCTTCCAGATTGCTATAAAATTA
AGCTTAAATCGTCCGGCTATCGTCTGGTGTACCAGGTTCGGGATGAGGTTGTGATCGTGTTTGTTGTCGCGGTCGGCAAG
CGAGAACATTCGGCCGTTTATCTCGATGCAAACAAACGGCTTTAG

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9331.64 Da
• Isoelectric Point: 4.6509

>AT230674 WP_001223213.1 NZ_CP090402:c4155207-4154956 [Shigella sp. PIB]
MRITIKRWGNSSGMVIPNVVMKELNLRPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNATELSEQDVWGKSTPAGD
EIW

Nucleotide

• Length: 252 bp

>AT230674 NZ_CP125310:564356-564598 [Citrobacter freundii]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAATGAGGCTTATGCCGTGCTGGAAAAACTGAACATTACTCC
AACGGAAGCTGTGCGATTACTGTTTCAGTACGTCGCGGAAACGGGGCGCATGCCAGTGAAAACCGTCACCGTTAGCGACA
GCGAAGATACACTGATTCAGACAGTCCGGGAGAGGCTGGCCAGCCCGCAAAAAGGAATCAAGGTTAGTCTGGATGACTTA
TGA

Structures

Toxin

• AlphaFold DB: V0SXT4

Antitoxin

• AlphaFold DB: L4JJX7