Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 3916124..3916674 Replicon chromosome
Accession NZ_CP090402
Organism Shigella sp. PIB

Toxin (Protein)


Gene name ccdB Uniprot ID A0A244BU83
Locus tag LXH19_RS18550 Protein ID WP_001160962.1
Coordinates 3916124..3916438 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag LXH19_RS18555 Protein ID WP_000125566.1
Coordinates 3916441..3916674 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LXH19_RS18525 (3911799) 3911799..3913085 + 1287 WP_000800457.1 peptidylprolyl isomerase SurA -
LXH19_RS18530 (3913085) 3913085..3914074 + 990 WP_000241249.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
LXH19_RS18535 (3914071) 3914071..3914892 + 822 WP_001065381.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
LXH19_RS18540 (3914895) 3914895..3915272 + 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
LXH19_RS18545 (3915279) 3915279..3916127 + 849 WP_021556397.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
LXH19_RS18550 (3916124) 3916124..3916438 - 315 WP_001160962.1 CcdB family protein Toxin
LXH19_RS18555 (3916441) 3916441..3916674 - 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
LXH19_RS18560 (3916760) 3916760..3917239 - 480 WP_000624375.1 type 3 dihydrofolate reductase -
LXH19_RS18565 (3917431) 3917431..3919293 - 1863 WP_000377114.1 glutathione-regulated potassium-efflux system protein KefC -
LXH19_RS18570 (3919286) 3919286..3919816 - 531 WP_000600725.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
LXH19_RS18575 (3919924) 3919924..3921255 - 1332 WP_001183198.1 MFS transporter -
LXH19_RS18580 (3921314) 3921314..3921601 - 288 WP_000203741.1 ferredoxin-like protein FixX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11741.79 Da        Isoelectric Point: 9.0851

>T230670 WP_001160962.1 NZ_CP090402:c3916438-3916124 [Shigella sp. PIB]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFIRIRPPERLNTILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T230670 NZ_CP125299:c4140767-4140417 [Pseudalkalibacillus hwajinpoensis]
TTGATTGTCAAACGAGGTGATGTGTATTTTGCTGACCTTTCTCCTGTTGTAGGTTCTGAACAAGGTGGGGTAAGACCCGT
CTTAATTATTCAGAACGACATTGGGAATCGTTTTAGTCCTACTGTCATTGTTGCCGCAATTACTGCGCAAATCCAAAAAG
CTAAGCTGCCCACACATGTGGAAATTGATGCCAAGCGTTACGGGTTTGAACGTGATTCGGTTATCCTGCTAGAACAAATT
CGTACCATTGATAAACAGCGTCTAACTGATAAAATCACTCACCTTGATGAGGGTATGATGCAAAGAGTGCAAGAAGCTTT
GCAGATCAGCCTTGGACTCATTGATTTTTGA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT230670 WP_000125566.1 NZ_CP090402:c3916674-3916441 [Shigella sp. PIB]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT230670 NZ_CP125299:c4141056-4140772 [Pseudalkalibacillus hwajinpoensis]
ATGTTTGTGTCAGAGGCAAACAAAAAATCGATTGTTGTAACTCTCCCACAACATATTTTAAATGAGGTGGATGGTATTAT
TCAACAGGAACAGCTTGATCGTAACGAGTTCATATCACAGGCCACGACAATGTATATTCGCGAACGAAAGAAGCGACAAA
TTCGTGACGCCATGCGCCAGGGGTACATGGAGATGGCTAAGATTAACTTGAACCTTGCAGCAGAAGCCTTTCTTGTTGAG
GAGGAAGCAGAGCACACCGTGGACCGCTTAGTAAGCGGGGTGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A244BU83

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References