Detailed information of TA system TA230668

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 3459297..3459915
• Replicon: chromosome
• Accession: NZ_CP090402
• Organism: Shigella sp. PIB

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: H5UYE2
• Locus tag: LXH19_RS16430
• Protein ID: WP_001291435.1
• Coordinates: 3459697..3459915 (+)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: S1PTH5
• Locus tag: LXH19_RS16425
• Protein ID: WP_000344800.1
• Coordinates: 3459297..3459671 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LXH19_RS16415 (3454386)	3454386..3455579	+	1194	WP_001295833.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-
LXH19_RS16420 (3455602)	3455602..3458751	+	3150	WP_001132480.1	efflux RND transporter permease AcrB	-
LXH19_RS16425 (3459297)	3459297..3459671	+	375	WP_000344800.1	Hha toxicity modulator TomB	Antitoxin
LXH19_RS16430 (3459697)	3459697..3459915	+	219	WP_001291435.1	HHA domain-containing protein	Toxin
LXH19_RS16435 (3460087)	3460087..3460638	+	552	WP_000102564.1	maltose O-acetyltransferase	-
LXH19_RS16440 (3460754)	3460754..3461224	+	471	WP_000136192.1	YlaC family protein	-
LXH19_RS16445 (3461388)	3461388..3462938	+	1551	WP_024216672.1	cyclic-guanylate-specific phosphodiesterase PdeB	-
LXH19_RS16450 (3462980)	3462980..3463333	-	354	WP_000878140.1	DUF1428 domain-containing protein	-
LXH19_RS16460 (3463712)	3463712..3464023	+	312	WP_000409911.1	MGMT family protein	-
LXH19_RS16465 (3464054)	3464054..3464626	-	573	WP_000779831.1	YbaY family lipoprotein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-117)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8628.00 Da
• Isoelectric Point: 8.9008

>T230668 WP_001291435.1 NZ_CP090402:3459697-3459915 [Shigella sp. PIB]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Nucleotide

• Length: 219 bp

>T230668 NZ_CP125292:c3654156-3653410 [Bacillus subtilis]
ATGGTTTTATTCTTTCAGATCATGGTCTGGTGCATCGTGGCCGGACTGGGGTTATACGTGTATGCCACGTGGCGTTTCGA
AGCAAAGGTCAAAGAAAAAATGTCCGCCATCCGGAAAACTTGGTATTTGCTGTTTGTTCTGGGCGCTATGGTATACTGGA
CATATGAGCCCACTTCCCTATTTACCCACTGGGAACGGTATCTCATTGTCGCAGTCAGTTTTGCTTTGATTGATGCTTTT
ATCTTCTTAAGTGCATACGTCAAAAAACTGGCCGGCAGCGAGCTTGAAACAGACACAAGAGAAATTCTTGAAGAAAACAA
CGAAATGCTCCACATGTATCTCAATCGGCTGAAAACATACCAATACCTATTGAAAAACGAACCGATCCATGTTTATTATG
GAAGTATAGATGCTTATGCTGAAGGTATTGATAAGCTGCTGAAAACCTATGCTGATAAAATGAACTTAACGGCTTCTCTT
TGCCACTATTCGACACAGGCTGATAAAGACCGGTTAACCGAGCATATGGATGATCCGGCAGATGTACAAACACGGCTCGA
TCGAAAGGATGTTTATTACGACCAATACGGAAAAGTGGTTCTCATCCCTTTTACCATCGAGACACAGAACTATGTCATCA
AGCTGACGTCTGACAGCATTGTCACGGAATTTGATTATTTGCTATTTACGTCATTAACGAGCATATATGATTTGGTGTTG
CCAATTGAGGAGGAAGGTGAAGGATAA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14557.39 Da
• Isoelectric Point: 4.7395

>AT230668 WP_000344800.1 NZ_CP090402:3459297-3459671 [Shigella sp. PIB]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Nucleotide

• Length: 375 bp

>AT230668 NZ_CP125292:c3653410-3653240 [Bacillus subtilis]
ATGGAACGTGCGTTTCAAAACAGATGCGAGCCCAGAGCGGCGAAGCCGTTTAAAATCCTGAAAAAACGTTCAACCACCAG
TGTCGCAAGCTATCAAGTCAGTCCGCATACAGCAAGAATCTTCAAAGAAAACGAACGGCTGATTGACGAGTATAAACGAA
AAAAAGCATGA

Structures

Toxin

• PDB: 2MW2
• PDB: 1JW2

Antitoxin

• AlphaFold DB: A0A7U9QBQ5