Detailed information of TA system TA230666

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2351992..2353580
• Replicon: chromosome
• Accession: NZ_CP090402
• Organism: Shigella sp. PIB

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: U9YVT8
• Locus tag: LXH19_RS11285
• Protein ID: WP_001125415.1
• Coordinates: 2352258..2353580 (+)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A366Y5V8
• Locus tag: LXH19_RS11280
• Protein ID: WP_001366357.1
• Coordinates: 2351992..2352258 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LXH19_RS11280 (2351992)	2351992..2352258	+	267	WP_001366357.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
LXH19_RS11285 (2352258)	2352258..2353580	+	1323	WP_001125415.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
LXH19_RS11290 (2353978)	2353978..2354877	-	900	WP_024216268.1	LysR family transcriptional regulator	-
LXH19_RS11295 (2355017)	2355017..2355325	+	309	WP_024044634.1	hypothetical protein	-
LXH19_RS11300 (2355334)	2355334..2356023	+	690	WP_000801313.1	type 1 glutamine amidotransferase domain-containing protein	-
LXH19_RS11305 (2356097)	2356097..2357044	+	948	WP_000854796.1	aldo/keto reductase	-
LXH19_RS11310 (2357147)	2357147..2357302	+	156	WP_001322415.1	hypothetical protein	-
LXH19_RS11315 (2357262)	2357262..2357825	+	564	WP_077688251.1	amidohydrolase family protein	-
LXH19_RS11320 (2357976)	2357976..2358548	+	573	WP_024044636.1	NAD(P)H-dependent oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (18-69)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49384.05 Da
• Isoelectric Point: 8.5830

>T230666 WP_001125415.1 NZ_CP090402:2352258-2353580 [Shigella sp. PIB]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLANRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVTHPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGNVRALAVERFDRRW
NAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIARIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGAGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLKFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVETNVLRLHRRLSREYGIK

Nucleotide

• Length: 1323 bp

>T230666 NZ_CP125291:c1583114-1582935 [Streptococcus pneumoniae]
ATGCCTATGACACAAAAAGAGATGGTTAAACTCCTTACGGCCCATGGTTGGATAAAGACTAGAGGCGGTAAAGGATCCCA
TATTAAAATGGAAAAGCAAGGGGAAAGACCTATCACAATCCTTCATGGTGAGCTGAATAAGTACACTGAAAGAGGGATAA
GAAAGCAAGCTGGGTTGTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10077.48 Da
• Isoelectric Point: 4.9632

>AT230666 WP_001366357.1 NZ_CP090402:2351992-2352258 [Shigella sp. PIB]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTTLMTFFKILQSLELSMTLCDTKNASPES
TEQQDLEW

Nucleotide

• Length: 267 bp

>AT230666 NZ_CP125291:c1582898-1582446 [Streptococcus pneumoniae]
ATGTTAGTTACGTATCCAGCTTTATTTTATTATGACGACACAGACGGAACAGAAGCGACTTATTTTGTCCATTTCCCAGA
TTTTGAATACTCAGCTACACAAGGAGAGGGGATTTCTGAGGCTTTGGCTATGGGGTCGGAGTGGCTAGGGATAACTGTTG
CAGATTTGATAGAGAGTGATGGAGAGTTACCTCAGCCATCAGATATCAATAGCTTGTCTTTAATTGATAATGATCCTTTT
AAAGATGATGAAGATTTCGTGTCAACCTATGACCTTGATAAATCTTTTATTTCTATGGTATCTGTTGATGTATCAGAATA
CCTAGGAAGTCAGGAACCCATTAAAAAGACTTTGACCATACCAAAATGGGCAGATAAGTTGGGACGAGAAATGGGGCTTA
ACTTTTCTCAGACTTTGACAGACGCTATTGCAGATAAGAAAGTTCAAGCCTAA

Structures

Toxin

• AlphaFold DB: U9YVT8

Antitoxin

• AlphaFold DB: A0A366Y5V8