Detailed information of TA system TA230652

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 158197..158809
• Replicon: chromosome
• Accession: NZ_CP090402
• Organism: Shigella sp. PIB

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: U9YXE2
• Locus tag: LXH19_RS00695
• Protein ID: WP_000833473.1
• Coordinates: 158197..158382 (+)
• Length: 62 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: L4IWR9
• Locus tag: LXH19_RS00700
• Protein ID: WP_000499744.1
• Coordinates: 158399..158809 (+)
• Length: 137 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LXH19_RS00680 (153714)	153714..154865	+	1152	WP_000741506.1	L-threonine dehydrogenase	-
LXH19_RS00685 (155066)	155066..156604	+	1539	WP_001469350.1	aldehyde dehydrogenase AldB	-
LXH19_RS00690 (156645)	156645..157724	-	1080	WP_000061477.1	type I restriction enzyme HsdR N-terminal domain-containing protein	-
LXH19_RS00695 (158197)	158197..158382	+	186	WP_000833473.1	type II toxin-antitoxin system HicA family toxin	Toxin
LXH19_RS00700 (158399)	158399..158809	+	411	WP_000499744.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
LXH19_RS00705 (158881)	158881..160845	-	1965	WP_024216364.1	glycoside hydrolase family 127 protein	-
LXH19_RS00710 (160856)	160856..162256	-	1401	WP_024216365.1	MFS transporter	-
LXH19_RS00715 (162482)	162482..163297	+	816	WP_000891838.1	AraC family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-129)

Sequences

Toxin

Protein

• Length: 62 a.a.
• Molecular weight: 6800.89 Da
• Isoelectric Point: 11.7053

>T230652 WP_000833473.1 NZ_CP090402:158197-158382 [Shigella sp. PIB]
VKSADVIAILKQHGWEHIRTRGSHHQFRHPVHPGLVTVPHPKKDIKPGTLAQIGRQAGLTF

Nucleotide

• Length: 186 bp

>T230652 NZ_CP125283:477345-477695 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAAAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 137 a.a.
• Molecular weight: 15241.15 Da
• Isoelectric Point: 4.5486

>AT230652 WP_000499744.1 NZ_CP090402:158399-158809 [Shigella sp. PIB]
MFYPAYIHSDPDGSASGFFPDVPGCYFAGDTLDNAFQDARSALVAHFETLCEMNEELPLPGSVETHLVQRAQDFIGGQWL
LVDINMNQFEGRAERINITMPKRLLNKIDTYVRNNPDYANRSAFLAEAARRVLPGV

Nucleotide

• Length: 411 bp

>AT230652 NZ_CP125283:477058-477339 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCTGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• AlphaFold DB: U9YXE2

Antitoxin

• AlphaFold DB: L4IWR9