Detailed information of TA system TA023065

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapB(antitoxin)
• Location: 216318..216886
• Replicon: chromosome
• Accession: NC_011206
• Organism: Acidithiobacillus ferrooxidans ATCC 53993

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: LFERR_RS01110
• Protein ID: WP_012535899.1
• Coordinates: 216318..216692 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: LFERR_RS14735
• Protein ID: WP_012535900.1
• Coordinates: 216689..216886 (-)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LFERR_RS01100	213578..214765	-	1188	WP_012535897.1	restriction endonuclease subunit S	-
LFERR_RS01105	214762..216216	-	1455	WP_012535898.1	type I restriction-modification system subunit M	-
LFERR_RS01110	216318..216692	-	375	WP_012535899.1	type II toxin-antitoxin system VapC family toxin	Toxin
LFERR_RS14735	216689..216886	-	198	WP_012535900.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
LFERR_RS01120	217862..218302	+	441	WP_012535902.1	1-acyl-sn-glycerol-3-phosphate acyltransferase	-
LFERR_RS01125	218478..219050	-	573	WP_012535903.1	PIN domain-containing protein	-
LFERR_RS01130	219051..219515	-	465	WP_012535904.1	helix-turn-helix domain-containing protein	-
LFERR_RS14740	219512..219796	-	285	WP_080515396.1	TIR domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	187366..219515	32149

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-109)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-46)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13683.77 Da
• Isoelectric Point: 6.3136

>T23065 WP_012535899.1 NC_011206:c216692-216318 [Acidithiobacillus ferrooxidans ATCC 53993]
MILVDTSVWIDHFRSGDAHLSTLLDHAMVQTHDFIIGEIACGNLKNRQQTLYLLSCLPRCTATSPEETLFFIGRHGLMGR
GIGYIDASILASAMLVQANLWSRDRRLMALATELGCAYSPTASE

Nucleotide

• Length: 375 bp

>T23065 NC_011206:c216692-216318 [Acidithiobacillus ferrooxidans ATCC 53993]
TTGATTCTGGTCGATACCTCCGTCTGGATCGACCATTTCCGCTCTGGAGATGCACACCTCTCTACTCTGCTTGATCATGC
CATGGTGCAAACGCACGATTTCATCATCGGAGAAATCGCTTGCGGGAATCTCAAAAATCGGCAACAGACCTTATATCTGC
TCTCATGTTTACCCCGATGCACCGCCACCTCTCCAGAAGAAACCCTGTTTTTCATTGGGCGTCATGGACTGATGGGACGC
GGTATAGGCTATATTGACGCGAGTATCCTAGCCTCCGCCATGCTCGTGCAGGCCAATTTATGGTCCCGCGACAGACGTCT
CATGGCTCTGGCTACCGAACTTGGCTGCGCGTACTCACCGACAGCATCCGAATAG

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 7294.39 Da
• Isoelectric Point: 9.6248

>AT23065 WP_012535900.1 NC_011206:c216886-216689 [Acidithiobacillus ferrooxidans ATCC 53993]
MRTTINLDDELLAQARTLCGVEDRGALLKEALRALIERESARRLARLGGTEPQLQNIPRRREPAA

Nucleotide

• Length: 198 bp

>AT23065 NC_011206:c216886-216689 [Acidithiobacillus ferrooxidans ATCC 53993]
ATGCGAACGACAATCAACCTGGATGATGAGCTCCTGGCCCAAGCCAGGACGCTCTGTGGCGTAGAAGACCGTGGTGCCCT
CTTAAAAGAGGCCCTGCGTGCTTTGATTGAACGGGAAAGTGCCCGTCGCCTCGCTCGCTTGGGTGGAACAGAACCGCAGT
TACAGAACATTCCTCGCAGACGAGAGCCTGCCGCTTGA