Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5375899..5376524 Replicon chromosome
Accession NZ_CP090398
Organism Klebsiella pneumoniae strain F94

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag LZG48_RS26495 Protein ID WP_002882817.1
Coordinates 5375899..5376282 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag LZG48_RS26500 Protein ID WP_004150355.1
Coordinates 5376282..5376524 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LZG48_RS26480 (LZG48_26480) 5373265..5374167 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
LZG48_RS26485 (LZG48_26485) 5374164..5374799 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
LZG48_RS26490 (LZG48_26490) 5374796..5375725 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
LZG48_RS26495 (LZG48_26495) 5375899..5376282 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
LZG48_RS26500 (LZG48_26500) 5376282..5376524 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
LZG48_RS26505 (LZG48_26505) 5376729..5377646 + 918 WP_002882812.1 alpha/beta hydrolase -
LZG48_RS26510 (LZG48_26510) 5377660..5378601 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
LZG48_RS26515 (LZG48_26515) 5378646..5379083 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
LZG48_RS26520 (LZG48_26520) 5379080..5379940 - 861 WP_002882807.1 virulence factor BrkB family protein -
LZG48_RS26525 (LZG48_26525) 5379934..5380533 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T230632 WP_002882817.1 NZ_CP090398:c5376282-5375899 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T230632 NZ_CP125220:c2693396-2693293 [Salmonella enterica subsp. enterica serovar Enteritidis]
GGCAAGGCGATTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT230632 WP_004150355.1 NZ_CP090398:c5376524-5376282 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT230632 NZ_CP125220:2693291-2693436 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGC
ATTAATGCAGGCTAAATCGCCTTGCCCTTTAAGAATAGATGACGACGCCAGGTTTTCCAGTTTGCG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References