Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5171172..5171797 Replicon chromosome
Accession NZ_CP090397
Organism Klebsiella pneumoniae strain F4

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag LZG49_RS24870 Protein ID WP_002882817.1
Coordinates 5171172..5171555 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag LZG49_RS24875 Protein ID WP_004150355.1
Coordinates 5171555..5171797 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LZG49_RS24855 (5168538) 5168538..5169440 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
LZG49_RS24860 (5169437) 5169437..5170072 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
LZG49_RS24865 (5170069) 5170069..5170998 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
LZG49_RS24870 (5171172) 5171172..5171555 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
LZG49_RS24875 (5171555) 5171555..5171797 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
LZG49_RS24880 (5172002) 5172002..5172919 + 918 WP_004181612.1 alpha/beta hydrolase -
LZG49_RS24885 (5172933) 5172933..5173874 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
LZG49_RS24890 (5173919) 5173919..5174356 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
LZG49_RS24895 (5174353) 5174353..5175213 - 861 WP_004146232.1 virulence factor BrkB family protein -
LZG49_RS24900 (5175207) 5175207..5175806 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T230614 WP_002882817.1 NZ_CP090397:c5171555-5171172 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T230614 NZ_CP125089:7220-7636 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT230614 WP_004150355.1 NZ_CP090397:c5171797-5171555 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT230614 NZ_CP125089:6993-7223 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References