Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3971072..3971691 Replicon chromosome
Accession NZ_CP090397
Organism Klebsiella pneumoniae strain F4

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag LZG49_RS19240 Protein ID WP_002892050.1
Coordinates 3971473..3971691 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag LZG49_RS19235 Protein ID WP_002892066.1
Coordinates 3971072..3971446 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LZG49_RS19225 (3966224) 3966224..3967417 + 1194 WP_002892072.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
LZG49_RS19230 (3967440) 3967440..3970586 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
LZG49_RS19235 (3971072) 3971072..3971446 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
LZG49_RS19240 (3971473) 3971473..3971691 + 219 WP_002892050.1 HHA domain-containing protein Toxin
LZG49_RS19245 (3971850) 3971850..3972416 + 567 WP_002892030.1 maltose O-acetyltransferase -
LZG49_RS19250 (3972388) 3972388..3972528 - 141 WP_004147370.1 hypothetical protein -
LZG49_RS19255 (3972549) 3972549..3973019 + 471 WP_002892026.1 YlaC family protein -
LZG49_RS19260 (3972994) 3972994..3974445 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
LZG49_RS19265 (3974546) 3974546..3975244 + 699 WP_002892021.1 GNAT family protein -
LZG49_RS19270 (3975241) 3975241..3975381 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
LZG49_RS19275 (3975381) 3975381..3975644 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T230610 WP_002892050.1 NZ_CP090397:3971473-3971691 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T230610 NZ_CP125088:c4782886-4782602 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT230610 WP_002892066.1 NZ_CP090397:3971072-3971446 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT230610 NZ_CP125088:c4783118-4782876 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References