Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5210298..5210923 Replicon chromosome
Accession NZ_CP090393
Organism Klebsiella pneumoniae strain YZH2429

Toxin (Protein)


Gene name vapC Uniprot ID A0A483GDF6
Locus tag LY283_RS25530 Protein ID WP_004187928.1
Coordinates 5210298..5210681 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag LY283_RS25535 Protein ID WP_004150355.1
Coordinates 5210681..5210923 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LY283_RS25515 (5207664) 5207664..5208566 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
LY283_RS25520 (5208563) 5208563..5209198 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
LY283_RS25525 (5209195) 5209195..5210124 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
LY283_RS25530 (5210298) 5210298..5210681 - 384 WP_004187928.1 type II toxin-antitoxin system VapC family toxin Toxin
LY283_RS25535 (5210681) 5210681..5210923 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
LY283_RS25540 (5211117) 5211117..5212034 + 918 WP_004187929.1 alpha/beta hydrolase -
LY283_RS25545 (5212048) 5212048..5212989 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
LY283_RS25550 (5213034) 5213034..5213471 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
LY283_RS25555 (5213468) 5213468..5214328 - 861 WP_004146232.1 virulence factor BrkB family protein -
LY283_RS25560 (5214322) 5214322..5214921 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-110)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14407.64 Da        Isoelectric Point: 7.3178

>T230593 WP_004187928.1 NZ_CP090393:c5210681-5210298 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFTGIPGVVTPYEIHPE

Download         Length: 384 bp

>T230593 NZ_CP125085:c144046-143603 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAACAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
TGGGCACGCTATCGCGGCCGGCGCCGTCCTAGTGACGAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGAAAGCCGCTCTCTGCAGGGTAATTTCTTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT230593 WP_004150355.1 NZ_CP090393:c5210923-5210681 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT230593 NZ_CP125085:c144273-144043 [Klebsiella pneumoniae subsp. pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGCTCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A483GDF6

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References