Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 43741..44477 Replicon plasmid pYUYZH22127-2
Accession NZ_CP090391
Organism Klebsiella pneumoniae strain YZH22127

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag LZC40_RS27450 Protein ID WP_003026803.1
Coordinates 43741..44223 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag LZC40_RS27455 Protein ID WP_003026799.1
Coordinates 44211..44477 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LZC40_RS27430 39460..39855 - 396 WP_004143398.1 helix-turn-helix domain-containing protein -
LZC40_RS27435 39904..41250 - 1347 WP_077253535.1 ISNCY family transposase -
LZC40_RS27440 41476..42108 + 633 WP_001567369.1 hypothetical protein -
LZC40_RS27445 42137..43540 - 1404 WP_001567368.1 ISNCY-like element ISKpn21 family transposase -
LZC40_RS27450 43741..44223 - 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
LZC40_RS27455 44211..44477 - 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
LZC40_RS27460 44653..44907 - 255 WP_004152108.1 hypothetical protein -
LZC40_RS27465 44983..45240 - 258 WP_004152107.1 hypothetical protein -
LZC40_RS27470 45289..45492 - 204 WP_004152106.1 HHA domain-containing protein -
LZC40_RS27475 45526..45894 - 369 WP_230444826.1 hypothetical protein -
LZC40_RS27480 45938..46432 - 495 WP_004152104.1 DNA-binding protein -
LZC40_RS27485 46463..47038 - 576 WP_004152103.1 hypothetical protein -
LZC40_RS27490 47026..47295 - 270 WP_004152102.1 hypothetical protein -
LZC40_RS27495 47732..48421 + 690 WP_020315560.1 RES family NAD+ phosphorylase -
LZC40_RS27500 48453..49142 - 690 WP_004193995.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaKPC-2 - 1..171602 171602


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T230573 WP_003026803.1 NZ_CP090391:c44223-43741 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T230573 NZ_CP124968:c810348-809833 [Agrobacterium tumefaciens]
GTGGCTTTATCGGCCCCCGAACTGCTCACCGCGGCGCATGACGTTTCTGAATTCTCTTGTGGTACGCCGACGCTCGATCA
TTGGCTGAAGACTCGTGCCCTATCCAATCAGCAGAAGGGTTTTACCGTCGTACTCGTCGTACACGAGGCTGGGCGCGTGG
TTGGATACTACGGTCTGGCGCCGACCGCCGTAGTGCCGGCGATCCTGCCACGCTCGATCCGCACCGGCCAGCCGCCTGAC
CCCGTGCCTTGCCTTCTCCTCGGTCAGCTTGCCACGGATGAAGCCTGGGCCGGGCAGGGGATCGGGACCGGCCTTGTCAA
GCACGCGCTCCAGCGGTGTGTTCAGGCCGCGAATCTGATCGGAGGACGCGCTCTGATGGTCAACGCCATCGATGACGAAG
CCGCCCGCTTCTGGCAGCGTCGAGGCTTTATGTCCTCGAAGGACGACCCGCTCGTCCTCTTCCGCTCTATTGCAGACATC
GCCGCGTCCTTCGCTGAAGCCCATGCACCAGCCTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT230573 WP_003026799.1 NZ_CP090391:c44477-44211 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT230573 NZ_CP124968:c810651-810352 [Agrobacterium tumefaciens]
ATGGCCACCATCGTTGAACGCAAGGAATACCCGATCTCGATGCGCCTGCCGGAAGCGGACGTCGCAATGATCGACCGCGC
CGCCACCCTGCGCGGACGCTCCCGCACCGACTTTGTGCGGGACGCCGCCGTGCGCGCGGCCGAGGATGTGTTGATGGAAA
ACCGGTTGATCCGCATGAGCCCCGAGGGCTTTGCCGAATTCATGGATGTTCTCGCTGCCCCGGCGGCTCCGGTCGCCGAG
ATGGTGGAACTGGCGCAACGGCCGGCGCCTTGGGAAACCGGCTATGAGCCGAAGCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References