Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4597366..4598176 Replicon chromosome
Accession NZ_CP090389
Organism Klebsiella pneumoniae strain YZH22127

Toxin (Protein)


Gene name KacT Uniprot ID A0A483G813
Locus tag LZC40_RS22605 Protein ID WP_023279404.1
Coordinates 4597366..4597899 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag LZC40_RS22610 Protein ID WP_002887278.1
Coordinates 4597910..4598176 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LZC40_RS22600 (4596197) 4596197..4597318 + 1122 WP_002887282.1 cupin domain-containing protein -
LZC40_RS22605 (4597366) 4597366..4597899 - 534 WP_023279404.1 type II toxin-antitoxin system toxin KacT Toxin
LZC40_RS22610 (4597910) 4597910..4598176 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
LZC40_RS22615 (4598279) 4598279..4599712 - 1434 WP_004192214.1 Cu(+)/Ag(+) sensor histidine kinase -
LZC40_RS22620 (4599702) 4599702..4600385 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
LZC40_RS22625 (4600557) 4600557..4601942 + 1386 WP_004192218.1 efflux transporter outer membrane subunit -
LZC40_RS22630 (4601960) 4601960..4602304 + 345 WP_004192220.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19809.71 Da        Isoelectric Point: 6.2369

>T230567 WP_023279404.1 NZ_CP090389:c4597899-4597366 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDKNSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T230567 NZ_CP124967:442291-442581 [Agrobacterium tumefaciens]
GTGGGCTTTCGCCTGTCTCTGCTCGCGGAAGAAGACGTCATTCGAATAGCCGAAGAAGGCATCGCGATATTCGGGCTGGC
GCAGGCGCAAAAATATCACCGGGAACTCTATGAGATATTTGAGCTGATATCTCTCAACCCACGCATGGCACGCGAGCGGC
ACGAGTTGTTACCGCCGCTGCGTATCCATCGGTTCAGGACACACCTGGTTGTTTATGGCGTGGGAGCGGACGATGATGTC
TTGATCGTCCGCGTCCGTCACGCACATGAGGACTGGGTCAACGAGTCGTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT230567 WP_002887278.1 NZ_CP090389:c4598176-4597910 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT230567 NZ_CP124967:442037-442288 [Agrobacterium tumefaciens]
ATGGCGACCATGAACATATCGCTGCCTGCACCCATGAAAGAATGGGTTGAGGCCCAATCCAGGACAGGCCGTTATTCGAA
TGCGAGCGACTACGTGCGAGACCTGATCCGCAAGGATCAGGAGCGAGGCGAAAAAATCGCGGCGATGCAGCATTTTGTGG
ATGAGGGATTGCAGAGCGGTGCCGGGCATCGCTCGCAACAGGCCTTGTTCGCCGAGGCTGAAGCTCGTTCCATGAAACGC
AAGATCGGTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A483G813

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References