Detailed information of TA system TA230552

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 325771..326357
• Replicon: chromosome
• Accession: NZ_CP090389
• Organism: Klebsiella pneumoniae strain YZH22127

Toxin (Protein)

• Gene name: doc
• Uniprot ID: A0A486Q6W0
• Locus tag: LZC40_RS01510
• Protein ID: WP_020802489.1
• Coordinates: 325989..326357 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: W9B1V1
• Locus tag: LZC40_RS01505
• Protein ID: WP_004174006.1
• Coordinates: 325771..325992 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LZC40_RS01485 (321928)	321928..322854	+	927	WP_002920807.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
LZC40_RS01490 (322851)	322851..324128	+	1278	WP_004174005.1	branched chain amino acid ABC transporter permease LivM	-
LZC40_RS01495 (324125)	324125..324892	+	768	WP_002920803.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
LZC40_RS01500 (324894)	324894..325607	+	714	WP_004145133.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF	-
LZC40_RS01505 (325771)	325771..325992	+	222	WP_004174006.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
LZC40_RS01510 (325989)	325989..326357	+	369	WP_020802489.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
LZC40_RS01515 (326630)	326630..327946	+	1317	WP_004174008.1	sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB	-
LZC40_RS01520 (328053)	328053..328940	+	888	WP_002920792.1	sn-glycerol-3-phosphate ABC transporter permease UgpA	-
LZC40_RS01525 (328937)	328937..329782	+	846	WP_004145129.1	sn-glycerol-3-phosphate ABC transporter permease UgpE	-
LZC40_RS01530 (329784)	329784..330854	+	1071	WP_004150074.1	sn-glycerol-3-phosphate import ATP-binding protein UgpC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	319950..331591	11641

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (1-121)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-50)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13542.89 Da
• Isoelectric Point: 8.6410

>T230552 WP_020802489.1 NZ_CP090389:325989-326357 [Klebsiella pneumoniae]
MTLQIISAEEIIQFHDRLLRVTSGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Nucleotide

• Length: 369 bp

>T230552 NZ_CP124923:1847903-1848247 [Enterococcus faecalis]
ATGAAAAGTATCAAAGAGTTGGTGGAAGAATATGAAGTAGAGTTAGTTTTCGCTCCAATAAATAAACGCGCATGTTACGA
GCCAGTCAAAAGAATAATTTTCGTAAATCAAAATTTATCTATCGAAGAACAAGAAGAGTCTATATTCCATGAGTTCAAAC
ATGTTGTCTCCCATTCGGATTATATTGAGTTATATAAAATTCCTTCTTTTAGAAATAAGATGGAAGCTGAGGCAGATTAT
CATATGTTTAAATGTCTTATTGAAAAACATGACGGGCAATTTAATTATTCTAATGTGATTACTCATTACAATTTAAAGAT
GGGACAGGAAACTTATTTAAATTAA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8151.15 Da
• Isoelectric Point: 4.7769

>AT230552 WP_004174006.1 NZ_CP090389:325771-325992 [Klebsiella pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Nucleotide

• Length: 222 bp

>AT230552 NZ_CP124923:1847553-1847885 [Enterococcus faecalis]
ATGAATACTTACGAAATAATAAAAGACTTAGCTAAAGAAAAAGGAATTTCTATTAGACAATTGGAAATAAATTTTGGCTA
CTCTAATGGATATATCGGTAGTTGGAAAAGACAAACACCCAATTCTAATGAATTAAGTCGCTTAGCAGACTACTTTGATG
TATCTGTAGATTATCTATTGGGAAGAACTGATAATACTAAAGCAACTGATGAAAAAAAATCTGATGATTTAGATGATGTA
CTGGATAACGTCATGAGTTTTGACGGTGAACCACTTGATGATCATGACAGAGAAGTTATTCGTGCTTACTTAAAAGGAAG
ATTCGGAAAATAA

Structures

Toxin

• AlphaFold DB: A0A486Q6W0

Antitoxin

• AlphaFold DB: A0A5E5YJY7