Detailed information of TA system TA230551

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 12743..13390
• Replicon: plasmid unnamed1
• Accession: NZ_CP090388
• Organism: Vibrio cholerae strain GXFL1-4

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: LY388_RS19515
• Protein ID: WP_032468292.1
• Coordinates: 12743..12922 (+)
• Length: 60 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: LY388_RS19520
• Protein ID: WP_234116237.1
• Coordinates: 12962..13390 (+)
• Length: 143 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LY388_RS19505 (LY388_19505)	7987..8331	+	345	WP_032468291.1	plasmid mobilization relaxosome protein MobC	-
LY388_RS19510 (LY388_19510)	8325..12578	+	4254	WP_234116236.1	hypothetical protein	-
LY388_RS19515 (LY388_19515)	12743..12922	+	180	WP_032468292.1	type II toxin-antitoxin system HicA family toxin	Toxin
LY388_RS19520 (LY388_19520)	12962..13390	+	429	WP_234116237.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
LY388_RS19525 (LY388_19525)	13395..13574	+	180	WP_234116238.1	hypothetical protein	-
LY388_RS19530 (LY388_19530)	13588..13791	+	204	WP_148521236.1	hypothetical protein	-
LY388_RS19535 (LY388_19535)	13795..14019	+	225	WP_148521238.1	hypothetical protein	-
LY388_RS19540 (LY388_19540)	14023..14184	+	162	WP_175247858.1	hypothetical protein	-
LY388_RS19545 (LY388_19545)	14353..14580	+	228	WP_148521240.1	hypothetical protein	-
LY388_RS19550 (LY388_19550)	14594..15070	+	477	WP_148521242.1	hypothetical protein	-
LY388_RS19555 (LY388_19555)	15057..15275	+	219	WP_234116239.1	hypothetical protein	-
LY388_RS19560 (LY388_19560)	15286..15471	+	186	WP_234116240.1	hypothetical protein	-
LY388_RS19565 (LY388_19565)	15485..15757	+	273	WP_234116241.1	hypothetical protein	-
LY388_RS19570 (LY388_19570)	15774..16124	+	351	WP_234116242.1	hypothetical protein	-
LY388_RS19575 (LY388_19575)	16109..16474	+	366	WP_234116243.1	hypothetical protein	-
LY388_RS19580 (LY388_19580)	16815..17354	-	540	WP_042989451.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..48351	48351

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-128)

Sequences

Toxin

Protein

• Length: 60 a.a.
• Molecular weight: 6599.83 Da
• Isoelectric Point: 11.2336

>T230551 WP_032468292.1 NZ_CP090388:12743-12922 [Vibrio cholerae]
VNSRELIKELEAAGWVLVRVKGSHHQFKHPHHKLPLTVPHPKKDLGVGLVKAIRKQAGL

Nucleotide

• Length: 180 bp

>T230551 NZ_CP124923:596747-596863 [Enterococcus faecalis]
ATGTTTTTGTCCGTTGAAGCAGCGCTAGGACTGATGATTGGTTTTGCAACACTTGTTGTGACCATTATCTTCGGTATCTT
AGCGCTTGTCTTAGACAACAAAAATAACCGTTCATAA

Antitoxin

Protein

• Length: 143 a.a.
• Molecular weight: 15610.69 Da
• Isoelectric Point: 4.4001

>AT230551 WP_234116237.1 NZ_CP090388:12962-13390 [Vibrio cholerae]
MLYPVAIEKGNDNSAFGAVFPDVAGCFAAGDSYEEALQNAREALELHFEALADMGEVPPSAATLDDHFNKPEFEGFLWAL
VDIDIEPYLGGAVKKNVTLPKLLLKKIDDLVKENPVYKDRSHFLQVAASREVDAVSMKQQRG

Nucleotide

• Length: 429 bp

>AT230551 NZ_CP124923:c596979-596774 [Enterococcus faecalis]
AAAAGAGAGATATGCAGGAACATACCTTTCTAATAGCCACTACTAGTTATAAGAACTAGCGGCTTACTGAGTTATTGGTT
TTATTTCAAACGCTTAACCGTTCAGCTCCTCAAAGCTTATGAACGGTTATTTTTGTTGTCTAAGACAAGCGCTAAGATAC
CGAAGATAATGGTCACAACAAGTGTTGCAAAACCAATCATCAGTCC