Detailed information of TA system TA230544

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 960257..960785
• Replicon: chromosome
• Accession: NZ_CP090386
• Organism: Vibrio cholerae strain GXFL1-4

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A0X1KZS6
• Locus tag: LY388_RS04185
• Protein ID: WP_000221354.1
• Coordinates: 960257..960544 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: LY388_RS04190
• Protein ID: WP_001250177.1
• Coordinates: 960534..960785 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LY388_RS04155 (LY388_04155)	956248..957174	-	927	WP_057563834.1	PD-(D/E)XK nuclease superfamily protein	-
LY388_RS04160 (LY388_04160)	957443..957739	-	297	Protein_828	DUF3709 domain-containing protein	-
LY388_RS04165 (LY388_04165)	957962..958249	-	288	WP_000869987.1	type II toxin-antitoxin system RelE/ParE family toxin	-
LY388_RS04170 (LY388_04170)	958246..958527	-	282	WP_000086649.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
LY388_RS04175 (LY388_04175)	958735..959199	-	465	WP_057563827.1	ASCH domain-containing protein	-
LY388_RS04180 (LY388_04180)	959350..960102	-	753	WP_001035644.1	hypothetical protein	-
LY388_RS04185 (LY388_04185)	960257..960544	-	288	WP_000221354.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LY388_RS04190 (LY388_04190)	960534..960785	-	252	WP_001250177.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
LY388_RS04195 (LY388_04195)	961092..961469	+	378	WP_000734783.1	PEGA domain-containing protein	-
LY388_RS04200 (LY388_04200)	961650..961757	-	108	WP_080391616.1	Tfp pilus assembly protein	-
LY388_RS04205 (LY388_04205)	961741..961863	-	123	WP_057569709.1	DUF645 family protein	-
LY388_RS04210 (LY388_04210)	962218..962397	-	180	WP_199358724.1	DUF645 family protein	-
LY388_RS04215 (LY388_04215)	962697..963233	-	537	WP_033931679.1	GNAT family protein	-
LY388_RS04220 (LY388_04220)	963383..963919	-	537	WP_000644495.1	nucleotidyltransferase family protein	-
LY388_RS04225 (LY388_04225)	964171..964407	+	237	WP_000772130.1	RNA-binding protein	-
LY388_RS04230 (LY388_04230)	964582..964941	-	360	WP_001071521.1	VOC family protein	-
LY388_RS04235 (LY388_04235)	965117..965716	-	600	WP_057563813.1	outer membrane beta-barrel protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integron	-	-	940330..981769	41439

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (11-70)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 10991.96 Da
• Isoelectric Point: 10.4934

>T230544 WP_000221354.1 NZ_CP090386:c960544-960257 [Vibrio cholerae]
MTYKLKFLPAAQKEWSKLAPTIQSQFKKKLKERLENPHVPSAKLRGYDAVYKIKLRTAGYRLAYEVIDDEIVVYVLAVGK
RDKDAVYKKLASRFG

Nucleotide

• Length: 288 bp

>T230544 NZ_CP124921:c2701008-2700865 [Enterococcus faecalis]
ATGAGCGTACTTCCAAAATTTACAGAAAGGAGAGGCCTGTTGTCAGCATATGAAACAATTCAGACGATCCTAGGGTTTGG
TATGTTTACCATTGCTTTGATTGCGCTGATTGTGAAATTGCTTAAAAATGACAATAAAAAATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9136.29 Da
• Isoelectric Point: 4.0197

>AT230544 WP_001250177.1 NZ_CP090386:c960785-960534 [Vibrio cholerae]
MRQVLANCSASISELKKNPTALLNEADGAAIAILNHNKPAAYLVPAETYEYLIDMLDDYELSQIVDSRRADLSQAVEVNI
DDL

Nucleotide

• Length: 252 bp

>AT230544 NZ_CP124921:2700746-2700932 [Enterococcus faecalis]
TGTGCTATAATGAAAACGAAAAGAGAGATATGCGTCAACATACCTCTCTGATGTAGAGCCGTTTAAGACGGTGACCGATT
TTGTTACAAAAAATAACCGTACTCGATCAAAGTAAACGGTTATTTTTTATTGTCATTTTTAAGCAATTTCACAATCAGCG
CAATCAAAGCAATGGTAAACATACCAA

Structures

Toxin

• AlphaFold DB: A0A0X1KZS6