230539

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) BrnTA/BrnT-BrnA
Location 33989..34577 Replicon plasmid pNDM_SCLZS30
Accession NZ_CP090384
Organism Acinetobacter towneri strain SCLZS30

Toxin (Protein)


Gene name brnT Uniprot ID N9CR77
Locus tag LZG54_RS14280 Protein ID WP_004971241.1
Coordinates 34290..34577 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name brnA Uniprot ID -
Locus tag LZG54_RS14275 Protein ID WP_234115058.1
Coordinates 33989..34303 (-) Length 105 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LZG54_RS14250 (LZG54_14265) 29354..30196 + 843 WP_002002480.1 carbapenem-hydrolyzing class D beta-lactamase OXA-58 -
LZG54_RS14255 (LZG54_14270) 30287..30993 + 707 Protein_29 IS1 family transposase -
LZG54_RS14260 (LZG54_14275) 31189..32049 + 861 WP_000557454.1 aminoglycoside N-acetyltransferase AAC(3)-IId -
LZG54_RS14265 (LZG54_14280) 32062..32604 + 543 WP_000587837.1 AAA family ATPase -
LZG54_RS14270 (LZG54_14285) 33195..33899 + 705 WP_001067858.1 IS6-like element IS26 family transposase -
LZG54_RS14275 (LZG54_14290) 33989..34303 - 315 WP_234115058.1 BrnA antitoxin family protein Antitoxin
LZG54_RS14280 (LZG54_14295) 34290..34577 - 288 WP_004971241.1 BrnT family toxin Toxin
LZG54_RS14285 (LZG54_14300) 34869..35510 - 642 WP_004787586.1 TetR/AcrR family transcriptional regulator C-terminal domain-containing protein -
LZG54_RS14290 (LZG54_14305) 35576..36763 + 1188 WP_004856455.1 tetracycline efflux MFS transporter Tet(39) -
LZG54_RS14295 (LZG54_14310) 36989..37600 + 612 WP_015060246.1 recombinase family protein -
LZG54_RS14300 (LZG54_14315) 37790..38674 - 885 WP_000155092.1 Mph(E) family macrolide 2'-phosphotransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaNDM-1 / aph(3')-VI / blaOXA-58 / aac(3)-IId / tet(39) / mph(E) / msr(E) - 1..47845 47845
- inside IScluster/Tn blaNDM-1 / aph(3')-VI / blaOXA-58 / aac(3)-IId / tet(39) / mph(E) / msr(E) - 20893..44160 23267


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(41-96)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11276.73 Da        Isoelectric Point: 6.0900

>T230539 WP_004971241.1 NZ_CP090384:c34577-34290 [Acinetobacter towneri]
MKQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRHTDGEERWQTIGKVKGVLMLLVAHTIFDEDDCEIIRIIS
ARQVTKAERDKYEHG

Download         Length: 288 bp

>T230539 NZ_CP124921:585384-585500 [Enterococcus faecalis]
ATGTTTTTGTCCGTTGAAGCAGCGTTGGGACTGATGATTGGTTTTGCAACACTTGTTGTGACCATTATCTTCGGTATCTT
AGCGCTTGTCTTAGACAACAAAAATAACCGTTCATAA

Antitoxin


Download         Length: 105 a.a.        Molecular weight: 12252.89 Da        Isoelectric Point: 7.2034

>AT230539 WP_234115058.1 NZ_CP090384:c34303-33989 [Acinetobacter towneri]
MSMVRYSRKELNEKFSEKQDAEIQRLLAKGTVPDDQLDLSDIPEITDWSNAIRHGQFYRPVKQQTSIRLDADVLAWFKAQ
GKGYQTRMNEILREAMLEDLKNHR

Download         Length: 315 bp

>AT230539 NZ_CP124921:c585616-585411 [Enterococcus faecalis]
AAAAGAGAGATATGCAGGAACATACCTCTCTAGTAGCCACTACTAGTTATAAGAACTAGCGGCTTACTGAGTTATTGGTT
TTATTTCAAACGCTTAACCGTTCAGCTCCTCAAAGCTTATGAACGGTTATTTTTGTTGTCTAAGACAAGCGCTAAGATAC
CGAAGATAATGGTCACAACAAGTGTTGCAAAACCAATCATCAGTCC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB N9CR77

Antitoxin

Source ID Structure

References