Detailed information of TA system TA230531

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-DnaT
• Location: 10117..10639
• Replicon: plasmid pBY369-1
• Accession: NZ_CP090380
• Organism: Vibrio cholerae strain BY369

Toxin (Protein)

• Gene name: relE
• Uniprot ID: G3CAN5
• Locus tag: LVJ30_RS18970
• Protein ID: WP_000220560.1
• Coordinates: 10358..10639 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: G3CAG1
• Locus tag: LVJ30_RS18965
• Protein ID: WP_000121743.1
• Coordinates: 10117..10368 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ30_RS18935 (LVJ30_18930)	5480..5710	+	231	WP_000051064.1	plasmid partition protein ParG	-
LVJ30_RS18940 (LVJ30_18935)	5726..6019	+	294	WP_015060510.1	hypothetical protein	-
LVJ30_RS18945 (LVJ30_18940)	6021..6989	+	969	WP_077783213.1	IS5-like element IS903B family transposase	-
LVJ30_RS18950 (LVJ30_18945)	7049..7402	-	354	WP_000876434.1	DNA distortion polypeptide 3	-
LVJ30_RS18955 (LVJ30_18950)	7539..7985	-	447	WP_001074386.1	hypothetical protein	-
LVJ30_RS18960 (LVJ30_18955)	8025..8861	-	837	WP_001050931.1	RepB family plasmid replication initiator protein	-
LVJ30_RS18965 (LVJ30_18960)	10117..10368	+	252	WP_000121743.1	plasmid stabilization protein	Antitoxin
LVJ30_RS18970 (LVJ30_18965)	10358..10639	+	282	WP_000220560.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LVJ30_RS18975 (LVJ30_18970)	10785..11108	+	324	WP_001181903.1	hypothetical protein	-
LVJ30_RS18980 (LVJ30_18975)	11153..11398	+	246	WP_000356546.1	hypothetical protein	-
LVJ30_RS18985 (LVJ30_18980)	11388..11603	+	216	WP_001180116.1	hypothetical protein	-
LVJ30_RS18990 (LVJ30_18985)	11696..12025	+	330	WP_000866648.1	hypothetical protein	-
LVJ30_RS18995 (LVJ30_18990)	12312..12461	+	150	WP_000003880.1	hypothetical protein	-
LVJ30_RS19000 (LVJ30_18995)	12474..12746	+	273	WP_000160399.1	hypothetical protein	-
LVJ30_RS19005 (LVJ30_19000)	13072..13617	+	546	WP_015060502.1	DNA distortion polypeptide 1	-
LVJ30_RS19010 (LVJ30_19005)	13620..14786	+	1167	WP_000539530.1	MobP1 family relaxase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaTEM-1B / aph(6)-Id / aph(3'')-Ib / tet(B)	mrkA / mrkB / mrkC / mrkD / mrkF / mrkJ	1..41043	41043
	-	flank	IS/Tn	-	-	6066..6989	923

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11005.83 Da
• Isoelectric Point: 10.5938

>T230531 WP_000220560.1 NZ_CP090380:10358-10639 [Vibrio cholerae]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADLR

Nucleotide

• Length: 282 bp

>T230531 NZ_CP124918:2807090-2807230 [Enterococcus faecalis]
ATTTCTCTGAAAAATACGAATAAAAATATCGTTTACTGTACAACATATGAAACAATTCAGACGATCCTAGGGTTTGGTAT
GTTTACCATTGCTTTGATTGCACTGATTGTGAAATTGCTTAAAAATGACAAGAAAAAATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9194.43 Da
• Isoelectric Point: 4.1683

>AT230531 WP_000121743.1 NZ_CP090380:10117-10368 [Vibrio cholerae]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL

Nucleotide

• Length: 252 bp

>AT230531 NZ_CP124918:c2807088-2806945 [Enterococcus faecalis]
TGCTACAATAGAGACGAAAAGAGAGGTATGCTCTAACATACCTCTCTAGTGTAGAGCCGTTTAAGACGGTGACCTTTTTA
GTTACAAAAAATAACCGTACTCAGTCAAAGTAGACGGTTATTTTTTCTTGTCATTTTTAAGCAA

Structures

Toxin

• AlphaFold DB: G3CAN5

Antitoxin

• AlphaFold DB: A0A3S5I301