Detailed information of TA system TA230495

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 3990393..3990983
• Replicon: chromosome
• Accession: NZ_CP090356
• Organism: Pantoea ananatis strain JBR-LB3-16

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: LVR30_RS18730
• Protein ID: WP_019106594.1
• Coordinates: 3990393..3990725 (-)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: LVR30_RS18735
• Protein ID: WP_014605554.1
• Coordinates: 3990726..3990983 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVR30_RS18705 (LVR30_18710)	3986872..3987126	-	255	WP_013026202.1	Arc family DNA-binding protein	-
LVR30_RS18710 (LVR30_18715)	3987200..3987904	-	705	WP_014605556.1	phosphohydrolase	-
LVR30_RS18715 (LVR30_18720)	3988516..3989193	+	678	WP_013026204.1	hypothetical protein	-
LVR30_RS18720 (LVR30_18725)	3989365..3990132	+	768	WP_227022858.1	DgsA anti-repressor MtfA	-
LVR30_RS18730 (LVR30_18735)	3990393..3990725	-	333	WP_019106594.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
LVR30_RS18735 (LVR30_18740)	3990726..3990983	-	258	WP_014605554.1	antitoxin	Antitoxin
LVR30_RS18740 (LVR30_18745)	3991331..3991537	-	207	WP_029570199.1	cell morphology transcriptional regulator XreR2	-
LVR30_RS18745 (LVR30_18750)	3991534..3991785	-	252	Protein_3646	hypothetical protein	-
LVR30_RS18750 (LVR30_18755)	3992037..3992999	-	963	WP_041930826.1	ferrochelatase	-
LVR30_RS18755 (LVR30_18760)	3993733..3994971	+	1239	WP_174260827.1	MFS transporter	-
LVR30_RS18760 (LVR30_18765)	3995263..3995688	-	426	WP_105079415.1	pentapeptide repeat-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-109)

Antitoxin

• Antitoxin PemI-like (4-83)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11931.63 Da
• Isoelectric Point: 6.9802

>T230495 WP_019106594.1 NZ_CP090356:c3990725-3990393 [Pantoea ananatis]
MDRGEIWLVSLDPNAGHEQSGRRPVLIVSPASFNQFTRLPVVVPVTNGGNFARAAGFTVSLDGAGTKTTGVIRCDQPRTI
DMEARNGKYLERIPEVIVNEVVARLEAILS

Nucleotide

• Length: 333 bp

>T230495 NZ_CP124911:c2732226-2732125 [Enterococcus faecalis]
TTGTCAATCGAAGCGGCATTGGAATTGATGATTAGTTTTGCAGCGTTTGTTGCACTACTGATTTTCGGTATCCTTGAAGC
AACGAAAAACGATAAAAAATAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9253.73 Da
• Isoelectric Point: 4.5821

>AT230495 WP_014605554.1 NZ_CP090356:c3990983-3990726 [Pantoea ananatis]
MYTTRLKKVGGSVMLAVPPAVLKTLGLSTDSEVGMTIDSGCLIIEPQKRPRYSLEELLAQCDPHAEISEEGREWIDAPAV
GKELL

Nucleotide

• Length: 258 bp

>AT230495 NZ_CP124911:2732003-2732180 [Enterococcus faecalis]
AAAAGAGAGGTATGCGGGTACATACCTCTCTTTTATACCAGCGCCAGTTATAAGAACTGGTGGCTTACTGAGTTAAGTTT
TTATTACTGTTTAACCGTTCGGCCTGTCAAGCTTATGAACGGTTATTTTTTATCGTTTTTCGTTGCTTCAAGGATACCGA
AAATCAGTAGTGCAACAA