Detailed information of TA system TA230485

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 3558147..3559495
• Replicon: chromosome
• Accession: NZ_CP090356
• Organism: Pantoea ananatis strain JBR-LB3-16

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: LVR30_RS16635
• Protein ID: WP_250331036.1
• Coordinates: 3558147..3559256 (-)
• Length: 370 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: LVR30_RS16640
• Protein ID: WP_013025828.1
• Coordinates: 3559256..3559495 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVR30_RS16625 (LVR30_16630)	3555365..3557233	+	1869	WP_013025825.1	low affinity potassium transporter Kup	-
LVR30_RS16630 (LVR30_16635)	3557685..3558101	+	417	WP_250331035.1	GFA family protein	-
LVR30_RS16635 (LVR30_16640)	3558147..3559256	-	1110	WP_250331036.1	HipA domain-containing protein	Toxin
LVR30_RS16640 (LVR30_16645)	3559256..3559495	-	240	WP_013025828.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
LVR30_RS16645 (LVR30_16650)	3559610..3560053	+	444	WP_013025829.1	GNAT family N-acetyltransferase	-
LVR30_RS16650 (LVR30_16655)	3560117..3562492	-	2376	WP_210459248.1	glycoside hydrolase family 3 N-terminal domain-containing protein	-
LVR30_RS16655 (LVR30_16660)	3562515..3563903	-	1389	WP_014605798.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (13-63)

Sequences

Toxin

Protein

• Length: 370 a.a.
• Molecular weight: 40930.26 Da
• Isoelectric Point: 7.6411

>T230485 WP_250331036.1 NZ_CP090356:c3559256-3558147 [Pantoea ananatis]
MAKLIAWINGERTGTLTLHHNGALHFQYDANWLQHPLARPLSLSLPLQRAAMHSEKVVHFFENLLYPSTLIEKDFYPTRP
IKSCEVFAALQKVGHTLPGALSLLPAKRQPVAPDQATHSVFSPADEAQQFDIDNAYLCLSLARELTIPVPDAVLVHENGI
RKLRTERYDRRWRHDGQHLYHLPEEDVCQALGVPLSGRDEQQGGPGIDDIMALLMGSSSAMKDREDMMRCLVFQWLTGIT
RVNAKHLSLLIARGGSFRLAPFKHLLSACPGLSLRTTDRRDFQLGMGLTGPEGKQHHIDAIGPAHFLALAGSVGFSQGSM
RQILAAFIPGLPAAIVKVRDSLPDDYPLTLADAVFSHAMMMLERVRQAI

Nucleotide

• Length: 1110 bp

>T230485 NZ_CP124908:c2812030-2811689 [Enterococcus faecalis]
ATGAATGTTGAAAAGAAGTACATTCCCAAAAAAGGAGATATTGTATGGATTGATTTTGATCCTGCTGCAGGCAAAGAGAT
TCAGAAGAGACGGCCTGGTTTAGTCGTGTCAAGATATGAATTTAATCGGAAAACCATGTTTGCTGTGATTTGCCCGATTA
CTTCCACCATTAAAAATATTCCCACGCGATATACGTTGCCCGATGACATAGAAACCCATGGACAAGTAGTGATCTCACAA
CTGAAATCTTTGGACTTCACTGAAAGAAAATTAAGTCAAATTGAGCATTTGCCATTAAAAGATATGGCGAAAATTGACCA
AATAATTGAATATATTTTCTAG

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8746.94 Da
• Isoelectric Point: 7.2012

>AT230485 WP_013025828.1 NZ_CP090356:c3559495-3559256 [Pantoea ananatis]
MIYSPRQLANQVRLLRQQNGWTQVELAKKVGLKQATLSHFENAPDTTSLATLFKILQALDLALSLHENSASSAPAEDAW

Nucleotide

• Length: 240 bp

>AT230485 NZ_CP124908:c2812260-2812030 [Enterococcus faecalis]
ATGCTAACAACTAAATCAAGAAGACAAGGAAGCTCGGTCGTTTTAACTTTACCCACTGATAATGGTAAAAAACCAAAAGT
CGACCAAGAATATATTGTGATGTATTCTGACGACGGTACAATTACATTAGTCCCTAAAATTCAGGATCCATTTAGTGAAG
GTCCAGAAGGTGAGTATTATGAAAAAGATGAGTGGCATGATCTTGCACCTGAGGGGAGAGAACTTTTTTAA