Detailed information of TA system TA230480

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 2271385..2272028
• Replicon: chromosome
• Accession: NZ_CP090355
• Organism: Pseudomonas kribbensis strain H38E-23

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A345RIP1
• Locus tag: LXN51_RS10550
• Protein ID: WP_065261145.1
• Coordinates: 2271385..2271567 (+)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: LXN51_RS10555
• Protein ID: WP_085684410.1
• Coordinates: 2271627..2272028 (+)
• Length: 134 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LXN51_RS10530 (LXN51_10530)	2266460..2267464	-	1005	WP_085702744.1	D-cysteine desulfhydrase	-
LXN51_RS10535 (LXN51_10535)	2267655..2268581	+	927	WP_085697995.1	serine acetyltransferase	-
LXN51_RS10540 (LXN51_10540)	2268870..2270831	+	1962	WP_176247724.1	choline transporter BetT	-
LXN51_RS10545 (LXN51_10545)	2270856..2271116	-	261	WP_085697997.1	hypothetical protein	-
LXN51_RS10550 (LXN51_10550)	2271385..2271567	+	183	WP_065261145.1	type II toxin-antitoxin system HicA family toxin	Toxin
LXN51_RS10555 (LXN51_10555)	2271627..2272028	+	402	WP_085684410.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
LXN51_RS10560 (LXN51_10560)	2272051..2272788	-	738	Protein_2063	substrate-binding domain-containing protein	-
LXN51_RS10565 (LXN51_10565)	2272792..2273886	+	1095	Protein_2064	TonB-dependent receptor	-
LXN51_RS10570 (LXN51_10570)	2273888..2275207	+	1320	WP_234206252.1	LLM class flavin-dependent oxidoreductase	-
LXN51_RS10575 (LXN51_10575)	2275247..2276284	+	1038	WP_234206254.1	L-glyceraldehyde 3-phosphate reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-127)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6836.96 Da
• Isoelectric Point: 10.5417

>T230480 WP_065261145.1 NZ_CP090355:2271385-2271567 [Pseudomonas kribbensis]
MRSREMIRMIEEDGWYLVAVKGSHHQYKHPGKPGRVTIKHPDSDLPKGTINSILKQAGLK

Nucleotide

• Length: 183 bp

>T230480 NZ_CP090355:2271385-2271567 [Pseudomonas kribbensis]
ATGCGTAGTCGGGAAATGATCAGGATGATCGAGGAGGATGGATGGTATCTGGTCGCCGTCAAAGGCAGTCACCACCAATA
CAAACATCCGGGCAAGCCAGGGAGGGTGACGATCAAGCATCCGGATTCAGATCTTCCCAAGGGAACGATCAACAGCATCT
TGAAACAGGCGGGTTTGAAATGA

Antitoxin

Protein

• Length: 134 a.a.
• Molecular weight: 14555.29 Da
• Isoelectric Point: 4.3652

>AT230480 WP_085684410.1 NZ_CP090355:2271627-2272028 [Pseudomonas kribbensis]
MKFPVVLHKDADSDYGVIIPDVPGCFSAGTTVAEAFDNTQEALALHYEGLVADGAPLPRVQDIDAHLENPDYAGGIWGVV
EFDITPYFGKSVRFNATLPEQLLERIDQTVRRDQRYSSRSGFLAAAALRELSA

Nucleotide

• Length: 402 bp

>AT230480 NZ_CP090355:2271627-2272028 [Pseudomonas kribbensis]
ATGAAATTCCCGGTCGTTCTGCACAAGGATGCCGACTCAGACTACGGAGTGATTATCCCGGATGTACCCGGGTGCTTCTC
CGCAGGCACTACGGTAGCCGAAGCATTTGACAACACTCAGGAGGCACTGGCCTTGCACTATGAAGGACTGGTCGCTGATG
GCGCACCGTTGCCTCGGGTTCAGGACATTGATGCCCATCTCGAGAATCCGGATTACGCCGGCGGTATCTGGGGTGTAGTC
GAGTTTGATATCACTCCCTACTTCGGCAAGTCGGTGCGCTTCAACGCTACGCTCCCGGAGCAGTTGCTGGAGCGTATTGA
CCAGACTGTGCGACGTGATCAGCGTTACAGCTCTCGCTCCGGATTTCTGGCTGCTGCTGCCTTGCGTGAATTGTCAGCTT
AA

Structures

Toxin

• AlphaFold DB: A0A345RIP1