Detailed information of TA system TA230477

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/ParE-CC2985
• Location: 17281..17819
• Replicon: plasmid unnamed3
• Accession: NZ_CP090352
• Organism: Methylobacterium oryzae strain H33R-06

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: LXM90_RS31460
• Protein ID: WP_234083696.1
• Coordinates: 17281..17571 (-)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: -
• Locus tag: LXM90_RS31465
• Protein ID: WP_058606647.1
• Coordinates: 17568..17819 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LXM90_RS31430 (LXM90_31430)	12959..13462	-	504	WP_234083680.1	multiubiquitin domain-containing protein	-
LXM90_RS31435 (LXM90_31435)	13574..13918	+	345	WP_234083683.1	helix-turn-helix transcriptional regulator	-
LXM90_RS31440 (LXM90_31440)	13921..14811	+	891	WP_234083686.1	ImmA/IrrE family metallo-endopeptidase	-
LXM90_RS31445 (LXM90_31445)	14955..15287	-	333	WP_234083689.1	hypothetical protein	-
LXM90_RS31450 (LXM90_31450)	15348..16010	-	663	WP_234083692.1	TetR/AcrR family transcriptional regulator	-
LXM90_RS31455 (LXM90_31455)	16064..17230	+	1167	WP_234083694.1	FAD-dependent monooxygenase	-
LXM90_RS31460 (LXM90_31460)	17281..17571	-	291	WP_234083696.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LXM90_RS31465 (LXM90_31465)	17568..17819	-	252	WP_058606647.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
LXM90_RS31470 (LXM90_31470)	18071..18835	+	765	WP_234083698.1	CHAP domain-containing protein	-
LXM90_RS31475 (LXM90_31475)	18849..19838	+	990	WP_234083700.1	serine protease	-
LXM90_RS31480 (LXM90_31480)	19873..20232	-	360	WP_234083702.1	hypothetical protein	-
LXM90_RS31485 (LXM90_31485)	20417..21169	+	753	WP_234083721.1	IS6 family transposase	-
LXM90_RS31490 (LXM90_31490)	21348..21992	-	645	WP_234083704.1	hypothetical protein	-
LXM90_RS31495 (LXM90_31495)	22237..22812	+	576	WP_234083706.1	recombinase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..49778	49778
	-	flank	IS/Tn	-	-	20582..21169	587

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

• Antitoxin ParD (1-79)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 10811.38 Da
• Isoelectric Point: 5.0484

>T230477 WP_234083696.1 NZ_CP090352:c17571-17281 [Methylobacterium oryzae]
MIFRTSPQADEDIADAYVAGALRFGAAQADRYQDGLFAVFQMLADNPLIARERRELIPPMRLHPYQAHMIVYVEEGAGVL
IVRVLHGRQDWESALA

Nucleotide

• Length: 291 bp

>T230477 NZ_CP124907:75891-76232 [Enterococcus faecalis]
ATGAGTGACGAAAAGAAATATATTCCTAAAAAGGGCGATATTGTATGGATAGATTTCGATCCTTCAGCTGAAAAAGAAAT
ACAGAAAAGACGTCCAGGTCTAGTAGTTTCTAGATATGAGTTTAATAGAAAGACATTGTTTGCAGTAATTTGTCCCATAA
CTTCTACAATTAAAAATATGCCAACTCGTTATACATTACCTGATGAAATGGAAACTCATGGACAAGTCGTTATTTCCCAA
TTAAAATCTTTAGACTTTGCAGAAAGAAAGTTAAGCCAAATAGAATATTTACCATTAAAGGATATGGCTAAAATAGATCA
AATCATTGAATACATTTTTTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9299.49 Da
• Isoelectric Point: 6.3109

>AT230477 WP_058606647.1 NZ_CP090352:c17819-17568 [Methylobacterium oryzae]
MATMNVSLPDPMKDWVEAQAGTGRYANASDYVRDLIRRDQDRLNKIAELQRLVDEGLASGISSRTPDEIRQLGRDRLAAI
QAK

Nucleotide

• Length: 252 bp

>AT230477 NZ_CP124907:75661-75891 [Enterococcus faecalis]
ATGTTAACCACAAAATCTAGAAAACAAGGAAGTTCTGTAGTATTGACATTACCATCTAATAACGGACAGAAACCAAAAGC
AGACCAAGAATATATAGTAATGTATTCTGATGATGGGACAATTACGTTAGTCCCTATATTACAAGACCCATTTAGTGGTG
GATCAGAAGGGGAGTACTACGAAAAAGATGAATGGCATGACATTGAACCTGGAGGGAGAGAATTATTTTAA