Detailed information of TA system TA230476

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/ParE-CC2985
• Location: 4202..4754
• Replicon: plasmid unnamed3
• Accession: NZ_CP090352
• Organism: Methylobacterium oryzae strain H33R-06

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: LXM90_RS31380
• Protein ID: WP_234083648.1
• Coordinates: 4202..4492 (-)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: -
• Locus tag: LXM90_RS31385
• Protein ID: WP_234083654.1
• Coordinates: 4485..4754 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LXM90_RS31365 (LXM90_31365)	264..1865	-	1602	WP_234083639.1	hypothetical protein	-
LXM90_RS31370 (LXM90_31370)	1885..2382	-	498	WP_234083642.1	hypothetical protein	-
LXM90_RS31375 (LXM90_31375)	2900..3715	-	816	WP_234083645.1	helix-turn-helix domain-containing protein	-
LXM90_RS31380 (LXM90_31380)	4202..4492	-	291	WP_234083648.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LXM90_RS31385 (LXM90_31385)	4485..4754	-	270	WP_234083654.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
LXM90_RS31390 (LXM90_31390)	5051..5701	+	651	WP_029360218.1	ParA family protein	-
LXM90_RS31395 (LXM90_31395)	5701..6048	+	348	WP_234083658.1	hypothetical protein	-
LXM90_RS31400 (LXM90_31400)	6365..6877	-	513	WP_234083661.1	hypothetical protein	-
LXM90_RS31405 (LXM90_31405)	6984..8579	+	1596	WP_234083664.1	restriction endonuclease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..49778	49778

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-90)

Antitoxin

• Antitoxin ParD (6-65)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 10651.18 Da
• Isoelectric Point: 7.5692

>T230476 WP_234083648.1 NZ_CP090352:c4492-4202 [Methylobacterium oryzae]
MSDYTLTWKAEADLFDIAVHGYRQFGVHQAEAYAAGLERAFQLLADNPGMGRKADAIRPGVHRHEHASHVILYEVVTGGV
LILAIIHGRSVRRFAL

Nucleotide

• Length: 291 bp

>T230476 NZ_CP124906:2639037-2639177 [Enterococcus faecalis]
ATTTCTCTGAAAAATACGAATAAAAATATCGTTTACTGTACAACATATGAAACAATTCAGACGATCCTAGGGTTTGGTAT
GTTTACCATTGCTTTGATTGCACTGATTGTGAAATTGCTTAAAAATGACAAGAAAAAATAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10130.47 Da
• Isoelectric Point: 7.0157

>AT230476 WP_234083654.1 NZ_CP090352:c4754-4485 [Methylobacterium oryzae]
MATMTISLPDPMKEWIEAQIKQGDYASTSDYVRDLVRRDRERRARPELTLEDLRQIVDDARASGPSGRKVPEILARAKKH
AQAAQPLDE

Nucleotide

• Length: 270 bp

>AT230476 NZ_CP124906:c2639365-2639110 [Enterococcus faecalis]
ATAAAAATGCCCGAGCCTCTTATTTTTCTAAGGCTCGGGCATTTTTATTGATGGTCTTTTGATGGTCGTTGTGCTACAAT
GGTAATGAAAAGAGAGCTATGCTGTAACATACCTCTCTGATGTAGAGCCGTTTAAGACGGTGACCTTTTTGATTATTTAA
AAATAACCGTACTGGTCAAAGTAGACGGTTATTTTTTCTTGTCATTTTTAAGCAATTTCACAATCAGTGCAATCAAAGCA
ATGGTAAACATACCAA