Detailed information of TA system TA230471

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /Xre(antitoxin)
• Location: 4485106..4486364
• Replicon: chromosome
• Accession: NZ_CP090348
• Organism: Pseudomonas aeruginosa strain PA8329

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: LXN03_RS20835
• Protein ID: WP_003163133.1
• Coordinates: 4485106..4485861 (-)
• Length: 252 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: V6AJU7
• Locus tag: LXN03_RS20840
• Protein ID: WP_003086329.1
• Coordinates: 4485996..4486364 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LXN03_RS20825 (LXN03_20825)	4480278..4482821	+	2544	WP_021205057.1	acyl-homoserine-lactone acylase QuiP	-
LXN03_RS20830 (LXN03_20830)	4482976..4484337	-	1362	WP_073654789.1	DNA recombination protein RmuC	-
LXN03_RS20835 (LXN03_20835)	4485106..4485861	-	756	WP_003163133.1	RES family NAD+ phosphorylase	Toxin
LXN03_RS20840 (LXN03_20840)	4485996..4486364	-	369	WP_003086329.1	DUF2384 domain-containing protein	Antitoxin
LXN03_RS20845 (LXN03_20845)	4486437..4487723	-	1287	WP_003163134.1	FAD-binding oxidoreductase	-
LXN03_RS20850 (LXN03_20850)	4487779..4489368	-	1590	WP_003123191.1	aldehyde dehydrogenase family protein	-
LXN03_RS20855 (LXN03_20855)	4489447..4489923	+	477	WP_004353334.1	elongation factor GreAB	-
LXN03_RS20860 (LXN03_20860)	4489926..4490201	-	276	WP_192929267.1	zinc ribbon domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (63-207)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-47)

Sequences

Toxin

Protein

• Length: 252 a.a.
• Molecular weight: 28250.37 Da
• Isoelectric Point: 7.7294

>T230471 WP_003163133.1 NZ_CP090348:c4485861-4485106 [Pseudomonas aeruginosa]
VSEIWRQCKGERHIRPLQGRLVRLVESQEQVATLQLVDTLEEQALLEELLESSKPPVPADAEPLHYLLKTPFRYPPLRWG
SRFGRRHEPSLFYAALKLETAMAESAYYRCVLWSGMAVPPPSGRILSEHASFEAGWKVERGIRLQAPPFSDHEAALTDIA
DYRAPQELGSAMRSAGVQAFEYRSARCPERGCNVALFTPAAFTEKRPRNLTPWLCETTAGYVAFKPAHVPGSPKIFSWEL
FLVDGKLPHPA

Nucleotide

• Length: 756 bp

>T230471 NZ_CP124906:c2488222-2488121 [Enterococcus faecalis]
TTGTCAATCGAAGCGGCATTGGAATTGATGATTAGTTTTGCAGCGTTTGTTGCACTACTGATTTTCGGTATCCTTGAAGC
AACGAAAAACGATAAAAAATAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13367.63 Da
• Isoelectric Point: 9.9874

>AT230471 WP_003086329.1 NZ_CP090348:c4486364-4485996 [Pseudomonas aeruginosa]
MSALIKERPSADAVLAKAVLAAREQLGLTQLELAGIVGVDRSAISRWKTQGLRVDSKTGELALLLVRVYRALYALFGGQQ
EDMRHFLRTPNHHLAGEPLALMGQVQGLVHVLEYLDAIRGKV

Nucleotide

• Length: 369 bp

>AT230471 NZ_CP124906:2487997-2488176 [Enterococcus faecalis]
TGAAAAGAGAGATATGCTACTACATACCTCTCCTTTATACCAACACCAGTTATAAGAACTGGTGGCTTACTGAGTTAAGT
TTTTGTTGTTCTTTAACCGTTCAGCCGTCTAAGCTTATGAACGGTTATTTTTTATCGTTTTTCGTTGCTTCAAGGATACC
GAAAATCAGTAGTGCAACAA

Structures

Antitoxin

• AlphaFold DB: V6AJU7