Detailed information of TA system TA230470

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/-
• Location: 3555083..3556304
• Replicon: chromosome
• Accession: NZ_CP090348
• Organism: Pseudomonas aeruginosa strain PA8329

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A0C7CS07
• Locus tag: LXN03_RS16450
• Protein ID: WP_003102740.1
• Coordinates: 3555726..3556304 (+)
• Length: 193 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: LXN03_RS16445
• Protein ID: WP_003088112.1
• Coordinates: 3555083..3555637 (+)
• Length: 185 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LXN03_RS16420 (LXN03_16420)	3550651..3551202	+	552	WP_003102727.1	XRE family transcriptional regulator	-
LXN03_RS16425 (LXN03_16425)	3551199..3551597	-	399	WP_003088123.1	NADH-quinone oxidoreductase subunit A	-
LXN03_RS16430 (LXN03_16430)	3551610..3551933	-	324	WP_003162861.1	multidrug efflux SMR transporter	-
LXN03_RS16435 (LXN03_16435)	3552264..3552725	+	462	WP_003102736.1	(2Fe-2S)-binding protein	-
LXN03_RS16440 (LXN03_16440)	3552730..3554925	+	2196	WP_004349295.1	xanthine dehydrogenase family protein molybdopterin-binding subunit	-
LXN03_RS16445 (LXN03_16445)	3555083..3555637	+	555	WP_003088112.1	helix-turn-helix transcriptional regulator	Antitoxin
LXN03_RS16450 (LXN03_16450)	3555726..3556304	+	579	WP_003102740.1	HD domain-containing protein	Toxin
LXN03_RS16455 (LXN03_16455)	3556413..3557600	-	1188	WP_003102741.1	HlyD family efflux transporter periplasmic adaptor subunit	-
LXN03_RS16460 (LXN03_16460)	3557590..3559761	-	2172	WP_003114950.1	type I secretion system permease/ATPase	-
LXN03_RS16465 (LXN03_16465)	3559751..3561028	-	1278	WP_003102743.1	TolC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HD domain (16-170)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-62)

Sequences

Toxin

Protein

• Length: 193 a.a.
• Molecular weight: 21869.70 Da
• Isoelectric Point: 5.3655

>T230470 WP_003102740.1 NZ_CP090348:3555726-3556304 [Pseudomonas aeruginosa]
MSLELLSNRLEFLREAERLKDVLRSAHTSSGRPESTAEHSWRLCLMALAFEDQLAGLDLGKVLRMCVVHDLGEAIHGDIP
AVEQAAHPDKGEQERADLLQLTRHLDAPLRDRLLALWDEYERGESAEALAVKALDKLETLLQHTQGDNPADFDYAFNLDY
GRRYTDRAPLFKTLRELLDARTRQRLAARRDA

Nucleotide

• Length: 579 bp

>T230470 NZ_CP124906:c2487789-2487688 [Enterococcus faecalis]
TTGTCAATCGAAGCGGCATTGGAATTGATGATTAGTTTTGCAGCGTTTGTTGCACTACTGATTTTCGGTATCCTTGAAGC
AACGAAAAACGATAAAAAATAA

Antitoxin

Protein

• Length: 185 a.a.
• Molecular weight: 20454.63 Da
• Isoelectric Point: 8.1590

>AT230470 WP_003088112.1 NZ_CP090348:3555083-3555637 [Pseudomonas aeruginosa]
MTEPLPSSSLGPALRRWRLLHRVKQTHAAELLGVAQSTISRWESGTQALLVEERARLERLLGARLEAAADQALARLVEAN
PQPVHLICDLTHRLLACSPARAAQFGVPLGELLDRSLWPYCSEEILRQEATLEELGWRELLAPPALEFASGANASAIVPI
RRSRCRWTRMTLSDGRAVRLVETL

Nucleotide

• Length: 555 bp

>AT230470 NZ_CP124906:2487566-2487743 [Enterococcus faecalis]
AAAAGAGAGGTATGCGGGTACATACCTCTCTTTTATACCAGCGCCAGTTATAAGAACTGGTGGCTTACTGAGTTAAGTTT
TTATTACTGTTTAACCGTTCGGCCTGTCAAGCTTATGAACGGTTATTTTTTATCGTTTTTCGTTGCTTCAAGGATACCGA
AAATCAGTAGTGCAACAA

Structures

Toxin

• AlphaFold DB: A0A0C7CS07