Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 108106..108749 Replicon plasmid pKPK3-1
Accession NZ_CP090344
Organism Klebsiella pneumoniae strain KPK3

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag LXL84_RS27355 Protein ID WP_001044770.1
Coordinates 108106..108522 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag LXL84_RS27360 Protein ID WP_001261282.1
Coordinates 108519..108749 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LXL84_RS27330 (LXL84_27295) 103424..103858 + 435 WP_000405672.1 Cd(II)/Pb(II)-responsive transcriptional regulator -
LXL84_RS27335 (LXL84_27300) 103949..104887 + 939 Protein_118 IS110 family transposase -
LXL84_RS27345 (LXL84_27310) 105622..106485 - 864 Protein_120 DNA helicase UvrD -
LXL84_RS27350 (LXL84_27315) 106470..108032 - 1563 WP_004206609.1 AAA family ATPase -
LXL84_RS27355 (LXL84_27320) 108106..108522 - 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
LXL84_RS27360 (LXL84_27325) 108519..108749 - 231 WP_001261282.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
LXL84_RS27365 (LXL84_27330) 108706..109122 + 417 WP_164481821.1 hypothetical protein -
LXL84_RS27370 (LXL84_27335) 109667..110488 + 822 WP_004213562.1 hypothetical protein -
LXL84_RS27375 (LXL84_27340) 110485..111264 + 780 WP_004213560.1 site-specific integrase -
LXL84_RS27380 (LXL84_27345) 111409..112338 - 930 WP_004213558.1 Rpn family recombination-promoting nuclease/putative transposase -
LXL84_RS27385 (LXL84_27350) 112642..112809 + 168 Protein_128 IS1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - rmpA2 / iutA / iucD / iucC / iucB / iucA / iroN 1..189486 189486
- inside IScluster/Tn - - 80890..105264 24374


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T230460 WP_001044770.1 NZ_CP090344:c108522-108106 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T230460 NZ_CP124902:c47937-47596 [Enterococcus faecalis]
ATGAGTGACGAAAAGAAATATATTCCTAAAAAGGGCGATATTGTATGGATAGATTTCGATCCTTCAGCTGGAAAAGAAAT
ACAGAAAAGACGTCCAGGTCTAGTAGTTTCTAGATATGAGTTTAATAGAAAGACAATGTTTGCAGTAATTTGTCCCATAA
CTTCTACAATTAAAAATATGCCCACTCGTTATACATTACCTGATGAAATGGAAACTCATGGACAAGTCGTTATTTCCCAA
TTAAAATCTTTAGATTTTACAGAAAGAAAGTTAAGCCAAATAGAACATTTACCATTAAAGGATATGGCTAAAATAGATCA
AATCATTGAATACATTTTTTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT230460 WP_001261282.1 NZ_CP090344:c108749-108519 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT230460 NZ_CP124902:c48167-47937 [Enterococcus faecalis]
ATGTTAACCACAAAATCTAGAAAACAAGGAAGTTCTGTAGTATTGACATTACCATCTAATAACGGACAGAAACCAAAAGC
AGACCAAGAATATGTAGTGATGTATTCTGATGATGGGACAATTACGTTAGTCCCTAAATTACAAGATCCATTTAGTGGTG
GATCAGAAGGGGAGTACTACGAAAAAGATGAATGGTATGACATTGAACCTGGAGGGAGAGAATTATTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References