Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) /RES-TIGR02293
Location 43598..44508 Replicon plasmid pKPK3-1
Accession NZ_CP090344
Organism Klebsiella pneumoniae strain KPK3

Toxin (Protein)


Gene name - Uniprot ID A0A663AYG0
Locus tag LXL84_RS27000 Protein ID WP_004026354.1
Coordinates 44038..44508 (+) Length 157 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A6P1V3Q9
Locus tag LXL84_RS26995 Protein ID WP_004026357.1
Coordinates 43598..44041 (+) Length 148 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LXL84_RS26970 (LXL84_26940) 39457..40023 + 567 WP_094818822.1 type I-E CRISPR-associated protein Cas6/Cse3/CasE -
LXL84_RS26975 (LXL84_26945) 40026..40988 + 963 WP_004902373.1 DEAD/DEAH box helicase -
LXL84_RS26980 (LXL84_26950) 41332..41553 - 222 WP_004213615.1 hypothetical protein -
LXL84_RS26985 (LXL84_26955) 41615..42340 - 726 Protein_48 TonB-dependent receptor -
LXL84_RS26990 (LXL84_26960) 42384..43364 + 981 WP_159205212.1 IS5-like element ISKpn26 family transposase -
LXL84_RS26995 (LXL84_26965) 43598..44041 + 444 WP_004026357.1 DUF2384 domain-containing protein Antitoxin
LXL84_RS27000 (LXL84_26970) 44038..44508 + 471 WP_004026354.1 RES family NAD+ phosphorylase Toxin
LXL84_RS27005 (LXL84_26975) 44657..45354 + 698 WP_223175001.1 IS1-like element IS1A family transposase -
LXL84_RS27010 (LXL84_26980) 45356..45805 + 450 WP_011251283.1 thioredoxin fold domain-containing protein -
LXL84_RS27015 (LXL84_26985) 45822..46133 + 312 WP_011251282.1 hypothetical protein -
LXL84_RS27020 (LXL84_26990) 46147..46488 + 342 WP_011251281.1 hypothetical protein -
LXL84_RS27025 (LXL84_26995) 46548..47504 + 957 WP_011251280.1 DsbA family protein -
LXL84_RS27030 (LXL84_27000) 47929..48369 - 441 WP_011251275.1 hypothetical protein -
LXL84_RS27035 (LXL84_27005) 48375..48869 - 495 WP_011251274.1 hypothetical protein -
LXL84_RS27040 (LXL84_27010) 48878..49192 - 315 WP_011251273.1 hypothetical protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - rmpA2 / iutA / iucD / iucC / iucB / iucA / iroN 1..189486 189486
- inside IScluster/Tn - iroN 38377..50789 12412


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(3-148)

Antitoxin

(30-89)


Sequences


Toxin        


Download         Length: 157 a.a.        Molecular weight: 17515.79 Da        Isoelectric Point: 4.6155

>T230458 WP_004026354.1 NZ_CP090344:44038-44508 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Download         Length: 471 bp

>T230458 NZ_CP124901:2740830-2740973 [Enterococcus faecalis]
ATGAATGTAAGTACTAAAATCTATGAAAGGAGAGGCCTTTTGTCTATCGCAGAAGCTTTAGCTCTGATGATTAGTTTCGG
TTCTTTTATCGCAACGTTAATCTTCGGAATCCTAGAGGCTGTTAAAGAAGACAATAAAAAATAA

Antitoxin


Download         Length: 148 a.a.        Molecular weight: 16480.80 Da        Isoelectric Point: 10.2498

>AT230458 WP_004026357.1 NZ_CP090344:43598-44041 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDKEAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Download         Length: 444 bp

>AT230458 NZ_CP124901:c2740736-2740535 [Enterococcus faecalis]
GTTTTTTTAGGTAATTGTGCTATAATAGCAATGAAAAGAGAGATATGCGCCAACATACCTCTCTGATGTAGAGCCGTTTA
AGACGGTGACCTTTTTGATTATTTAAAAATAACCGTACTGGTCAAAGTAGACGGTTATTTTTTCTTGTCATTTTTAAGCA
ATTTCACAATCAGCGCAATCAAAGCAATGGTAAACATACCAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A663AYG0


Antitoxin

Source ID Structure
AlphaFold DB A0A6P1V3Q9

References