Detailed information of TA system TA230457

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 11315..11985
• Replicon: plasmid pKPK3-1
• Accession: NZ_CP090344
• Organism: Klebsiella pneumoniae strain KPK3

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: LXL84_RS26805
• Protein ID: WP_004213072.1
• Coordinates: 11542..11985 (+)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: LXL84_RS26800
• Protein ID: WP_004213073.1
• Coordinates: 11315..11545 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LXL84_RS26775 (LXL84_26745)	7538..8437	-	900	WP_004225022.1	nucleotide-binding protein	-
LXL84_RS26780 (LXL84_26750)	8427..8717	-	291	WP_004213078.1	hypothetical protein	-
LXL84_RS26785 (LXL84_26755)	9069..9275	+	207	WP_004213077.1	hypothetical protein	-
LXL84_RS26790 (LXL84_26760)	9265..9558	-	294	WP_004213076.1	hypothetical protein	-
LXL84_RS26795 (LXL84_26765)	9574..10707	-	1134	WP_004213075.1	DUF3800 domain-containing protein	-
LXL84_RS26800 (LXL84_26770)	11315..11545	+	231	WP_004213073.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
LXL84_RS26805 (LXL84_26775)	11542..11985	+	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
LXL84_RS26810 (LXL84_26780)	12134..12385	+	252	WP_186987481.1	hypothetical protein	-
LXL84_RS26815 (LXL84_26785)	12408..12712	-	305	Protein_14	transposase	-
LXL84_RS26820 (LXL84_26790)	13129..13764	+	636	Protein_15	mucoid phenotype regulator RmpA2	-
LXL84_RS26825 (LXL84_26795)	14282..14685	-	404	Protein_16	GAF domain-containing protein	-
LXL84_RS26830 (LXL84_26800)	14776..15696	-	921	WP_004213934.1	DUF1471 domain-containing protein	-
LXL84_RS26835 (LXL84_26805)	15745..16236	-	492	WP_011251327.1	DM13 domain-containing protein	-
LXL84_RS26840 (LXL84_26810)	16299..16574	-	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	rmpA2 / iutA / iucD / iucC / iucB / iucA / iroN	1..189486	189486

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T230457 WP_004213072.1 NZ_CP090344:11542-11985 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T230457 NZ_CP124901:2740462-2740602 [Enterococcus faecalis]
ATTTCTCTGAAAAATACGAATAAAAATATCGTTTACTGTACAACATATGAAACAATTCAGACGATCCTAGGGTTTGGTAT
GTTTACCATTGCTTTGATTGCGCTGATTGTGAAATTGCTTAAAAATGACAAGAAAAAATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT230457 WP_004213073.1 NZ_CP090344:11315-11545 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT230457 NZ_CP124901:c2740736-2740535 [Enterococcus faecalis]
GTTTTTTTAGGTAATTGTGCTATAATAGCAATGAAAAGAGAGATATGCGCCAACATACCTCTCTGATGTAGAGCCGTTTA
AGACGGTGACCTTTTTGATTATTTAAAAATAACCGTACTGGTCAAAGTAGACGGTTATTTTTTCTTGTCATTTTTAAGCA
ATTTCACAATCAGCGCAATCAAAGCAATGGTAAACATACCAA

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620