Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5343474..5344099 Replicon chromosome
Accession NZ_CP090343
Organism Klebsiella pneumoniae strain KPK3

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag LXL84_RS26370 Protein ID WP_002882817.1
Coordinates 5343474..5343857 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag LXL84_RS26375 Protein ID WP_004150355.1
Coordinates 5343857..5344099 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LXL84_RS26355 (LXL84_26325) 5340840..5341742 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
LXL84_RS26360 (LXL84_26330) 5341739..5342374 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
LXL84_RS26365 (LXL84_26335) 5342371..5343300 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
LXL84_RS26370 (LXL84_26340) 5343474..5343857 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
LXL84_RS26375 (LXL84_26345) 5343857..5344099 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
LXL84_RS26380 (LXL84_26350) 5344304..5345221 + 918 WP_002882812.1 alpha/beta hydrolase -
LXL84_RS26385 (LXL84_26355) 5345235..5346176 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
LXL84_RS26390 (LXL84_26360) 5346221..5346658 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
LXL84_RS26395 (LXL84_26365) 5346655..5347515 - 861 WP_002882807.1 virulence factor BrkB family protein -
LXL84_RS26400 (LXL84_26370) 5347509..5348108 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T230456 WP_002882817.1 NZ_CP090343:c5343857-5343474 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T230456 NZ_CP124901:2740462-2740602 [Enterococcus faecalis]
ATTTCTCTGAAAAATACGAATAAAAATATCGTTTACTGTACAACATATGAAACAATTCAGACGATCCTAGGGTTTGGTAT
GTTTACCATTGCTTTGATTGCGCTGATTGTGAAATTGCTTAAAAATGACAAGAAAAAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT230456 WP_004150355.1 NZ_CP090343:c5344099-5343857 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT230456 NZ_CP124901:c2740460-2740317 [Enterococcus faecalis]
TGCTACAATAGAGACGAAAAGAGAGGTATGCTCTAACATACCTCTCTAGTGTAGAGCCGTTTAAGACGGTGACCTTTTTA
GTTACAAAAAATAACCGTACTCAGTCAAAGTAGACGGTTATTTTTTCTTGTCATTTTTAAGCAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References