Detailed information of TA system TA230429

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-dinJ/YafQ-RelB
• Location: 1601344..1601875
• Replicon: chromosome
• Accession: NZ_CP090316
• Organism: Xylella fastidiosa subsp. morus strain Riv16

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A060HEY7
• Locus tag: IUD23_RS07185
• Protein ID: WP_038230161.1
• Coordinates: 1601594..1601875 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: A0A060H9I0
• Locus tag: IUD23_RS07180
• Protein ID: WP_004086967.1
• Coordinates: 1601344..1601607 (+)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IUD23_RS07150 (IUD23_07150)	1596393..1597412	+	1020	WP_038230156.1	tetraacyldisaccharide 4'-kinase	-
IUD23_RS07155 (IUD23_07155)	1597828..1598505	-	678	WP_234056269.1	contractile injection system protein, VgrG/Pvc8 family	-
IUD23_RS07160 (IUD23_07160)	1598435..1599406	-	972	WP_234056270.1	phage tail protein	-
IUD23_RS07165 (IUD23_07165)	1599414..1599971	-	558	WP_027700436.1	phage tail protein I	-
IUD23_RS07170 (IUD23_07170)	1599964..1600857	-	894	WP_038230566.1	baseplate J/gp47 family protein	-
IUD23_RS07175 (IUD23_07175)	1600857..1601180	-	324	WP_228304176.1	GPW/gp25 family protein	-
IUD23_RS07180 (IUD23_07180)	1601344..1601607	+	264	WP_004086967.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
IUD23_RS07185 (IUD23_07185)	1601594..1601875	+	282	WP_038230161.1	type II toxin-antitoxin system YafQ family toxin	Toxin
IUD23_RS07190 (IUD23_07190)	1601912..1602499	-	588	WP_038230163.1	phage baseplate assembly protein V	-
IUD23_RS07195 (IUD23_07195)	1602496..1603038	-	543	WP_038230164.1	hypothetical protein	-
IUD23_RS07200 (IUD23_07200)	1603014..1603535	-	522	WP_038230165.1	hypothetical protein	-
IUD23_RS07205 (IUD23_07205)	1603532..1603855	-	324	WP_027700441.1	hypothetical protein	-
IUD23_RS07210 (IUD23_07210)	1603855..1604115	-	261	WP_027700442.1	hypothetical protein	-
IUD23_RS07215 (IUD23_07215)	1604133..1606006	-	1874	Protein_1403	phage major capsid protein	-
IUD23_RS07220 (IUD23_07220)	1606003..1606635	-	633	WP_040123076.1	phage portal protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1589994..1613025	23031

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (2-91)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (4-70)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10834.28 Da
• Isoelectric Point: 7.5505

>T230429 WP_038230161.1 NZ_CP090316:1601594-1601875 [Xylella fastidiosa subsp. morus]
MRRISQTGQFKRDYKREAKGQHRATLDEELIHVVEALACDHPLEPRHHDHALTGGWKDHRDCHIKPDLVLIYRKPDNETL
QLVRIGSHSELGL

Nucleotide

• Length: 282 bp

>T230429 NZ_CP124894:2852936-2853079 [Enterococcus faecalis]
ATGAATGTAAGTACTAAAATCTATGAAAGGAGAGGCCTTTTGTCTATCGCAGAAGCTTTAGCTCTGATGATTAGTTTCGG
TTCTTTTATCGCAACGTTAATCTTCGGAATCCTAGAGGCTGTTAAAGAAGACAATAAAAAATAA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 9525.98 Da
• Isoelectric Point: 5.6033

>AT230429 WP_004086967.1 NZ_CP090316:1601344-1601607 [Xylella fastidiosa subsp. morus]
MSANAVVRARVDTHIKEEASTVLATMGLSVSDAFRMMLTRIAREKALPFEPLVPNETTIAAMREARAGHLKSFDSIEALM
ADLHAED

Nucleotide

• Length: 264 bp

>AT230429 NZ_CP124894:c2853273-2853011 [Enterococcus faecalis]
AAATAAAAATGCCCGAGCCTCTTATTTTTCTAAGGCTCGGGCATTTTTATTGATGGTCTTTTGAGGGTCGTTGTGCTATA
ATGGTAATGAAAAGAGAGGTATGCGCCAACATACCTCTCTAGTGTAGAGCCGTTTCAGACGGTAGCGATTTTTAGTGATT
TATAAAAAATAACCGTGCTCGCTAAAGTTGACGGTTATTTTTTATTGTCTTCTTTAACAGCCTCTAGGATTCCGAAGATT
AACGTTGCGATAAAAGAACCGAA

Structures

Toxin

• AlphaFold DB: A0A060HEY7

Antitoxin

• AlphaFold DB: A0A060H9I0