Detailed information of TA system TA230427

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 971732..972318
• Replicon: chromosome
• Accession: NZ_CP090316
• Organism: Xylella fastidiosa subsp. morus strain Riv16

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: IUD23_RS04485
• Protein ID: WP_038228269.1
• Coordinates: 971732..971986 (+)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: B2I6C9
• Locus tag: IUD23_RS04490
• Protein ID: WP_004085025.1
• Coordinates: 971983..972318 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IUD23_RS04450 (IUD23_04450)	967439..968044	-	606	WP_040123201.1	conjugal transfer protein TrbN	-
IUD23_RS04455 (IUD23_04455)	968050..968424	-	375	WP_040123200.1	hypothetical protein	-
IUD23_RS04460 (IUD23_04460)	968571..968825	+	255	WP_040123199.1	hypothetical protein	-
IUD23_RS04465 (IUD23_04465)	968822..969724	+	903	WP_234056418.1	YdaU family protein	-
IUD23_RS04470 (IUD23_04470)	969705..970394	+	690	WP_038228265.1	hypothetical protein	-
IUD23_RS04475 (IUD23_04475)	970391..970852	+	462	WP_234056374.1	hypothetical protein	-
IUD23_RS04480 (IUD23_04480)	970981..971679	+	699	WP_038228267.1	hypothetical protein	-
IUD23_RS04485 (IUD23_04485)	971732..971986	+	255	WP_038228269.1	type II toxin-antitoxin system HicA family toxin	Toxin
IUD23_RS04490 (IUD23_04490)	971983..972318	+	336	WP_004085025.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
IUD23_RS04495 (IUD23_04495)	972498..972797	-	300	WP_004089519.1	HigA family addiction module antitoxin	-
IUD23_RS04500 (IUD23_04500)	972807..973088	-	282	WP_011098058.1	type II toxin-antitoxin system RelE/ParE family toxin	-
IUD23_RS04505 (IUD23_04505)	973436..974150	+	715	Protein_880	site-specific DNA-methyltransferase	-
IUD23_RS04510 (IUD23_04510)	974137..974601	-	465	WP_011098056.1	low molecular weight protein-tyrosine-phosphatase	-
IUD23_RS04515 (IUD23_04515)	974598..975371	-	774	WP_004089528.1	3-deoxy-manno-octulosonate cytidylyltransferase	-
IUD23_RS04520 (IUD23_04520)	975806..976717	+	912	WP_230577717.1	iron-sulfur cluster carrier protein ApbC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	952522..999251	46729

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (23-80)

Antitoxin

• Uncharacterised protein family HicB (55-105)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 9679.16 Da
• Isoelectric Point: 11.2766

>T230427 WP_038228269.1 NZ_CP090316:971732-971986 [Xylella fastidiosa subsp. morus]
MKRKHAKTLSAIYTRPVSANIQWRDIEALFVELGAKVEEREGSRMLVRLFGERRIFHRPHPSHNTDKGAVESIRAWLKSN
GVTP

Nucleotide

• Length: 255 bp

>T230427 NZ_CP124894:c2727612-2727469 [Enterococcus faecalis]
ATGAGCGTACTTCCAAAATTTACGGAAAGGAGAGGCCTTTTGTCAGCATATGAAACAATTCAGACGATCCTAGGGTTTGG
TATGTTTACCATTGCTTTGATTGCACTGATTGTGAAATTGCTTAAAAATGACAATAAAAAATAA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12324.07 Da
• Isoelectric Point: 5.6556

>AT230427 WP_004085025.1 NZ_CP090316:971983-972318 [Xylella fastidiosa subsp. morus]
MKNIMTIESFKAIITYDPEIDMFRGEFVGINGGADFYAKDLKGLRREGAISLKVFLEACKEDSVEPRKCYSGKFNARISP
ELHALASEAAAAQGISLNQFVEQAIQHEVHT

Nucleotide

• Length: 336 bp

>AT230427 NZ_CP124894:2727350-2727495 [Enterococcus faecalis]
TGTGCTATAATGAAAACGAAAAGAGAGATATGCTTCAACATACCTCTCTGATGTAGAGCCGTTTAAGACGGTGACCGATT
GTATTATTTAAAAATAACCGTACTGGTCAAAGTAGACGGTTATTTTTTATTGTCATTTTTAAGCAA

Structures

Antitoxin

• AlphaFold DB: A0A060HAK5