Detailed information of TA system TA230426

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/COG3657-dnstrm_HI1420
• Location: 534319..534924
• Replicon: chromosome
• Accession: NZ_CP090316
• Organism: Xylella fastidiosa subsp. morus strain Riv16

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: B2I5N3
• Locus tag: IUD23_RS02535
• Protein ID: WP_004089252.1
• Coordinates: 534628..534924 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: IUD23_RS02530
• Protein ID: WP_038211623.1
• Coordinates: 534319..534624 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IUD23_RS02490 (IUD23_02490)	530707..531015	-	309	WP_004085003.1	putative addiction module antidote protein	-
IUD23_RS02495 (IUD23_02495)	531019..531318	-	300	WP_024749166.1	type II toxin-antitoxin system RelE/ParE family toxin	-
IUD23_RS02500 (IUD23_02500)	531406..531711	+	306	WP_230577600.1	hypothetical protein	-
IUD23_RS02505 (IUD23_02505)	531727..532176	+	450	WP_230577599.1	hypothetical protein	-
IUD23_RS02510 (IUD23_02510)	532176..532493	+	318	WP_012382585.1	hypothetical protein	-
IUD23_RS02515 (IUD23_02515)	532490..533320	+	831	WP_234056377.1	hypothetical protein	-
IUD23_RS02520 (IUD23_02520)	533317..533958	+	642	WP_040123250.1	Gp138 family membrane-puncturing spike protein	-
IUD23_RS02525 (IUD23_02525)	533955..534308	+	354	WP_234056392.1	hypothetical protein	-
IUD23_RS02530 (IUD23_02530)	534319..534624	-	306	WP_038211623.1	putative addiction module antidote protein	Antitoxin
IUD23_RS02535 (IUD23_02535)	534628..534924	-	297	WP_004089252.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IUD23_RS02540 (IUD23_02540)	535023..536168	+	1146	WP_038211620.1	baseplate J/gp47 family protein	-
IUD23_RS02545 (IUD23_02545)	536165..536725	+	561	WP_011097869.1	DUF2612 domain-containing protein	-
IUD23_RS02550 (IUD23_02550)	536729..537892	+	1164	WP_040123248.1	tail fiber protein	-
IUD23_RS02555 (IUD23_02555)	538189..538341	+	153	WP_162531331.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	422869..537892	115023

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-94)

Antitoxin

• Cro/C1-type helix-turn-helix domain (50-93)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 10790.57 Da
• Isoelectric Point: 10.0509

>T230426 WP_004089252.1 NZ_CP090316:c534924-534628 [Xylella fastidiosa subsp. morus]
MVELIKTSTFDAWINSLRDRKAAARIQARLDRLALGNPGDVKPVGAGISEMRIDHGPGYRIYFMKHGAVLILLLCGGDKS
SQVRDIEQAKALAALWKD

Nucleotide

• Length: 297 bp

>T230426 NZ_CP124894:c2727237-2727094 [Enterococcus faecalis]
ATGAGCGTACTTCCAAAATTTACAGAAAGGAGAGGCCTGTTGTCAGCATATGAAACAATTCAGACGATCCTAGGGTTTGG
TATGTTTACCATTGCTTTGATTGCGCTGATTGTGAAATTGCTTAAAAATGACAATAAAAAATAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 10982.42 Da
• Isoelectric Point: 5.0941

>AT230426 WP_038211623.1 NZ_CP090316:c534624-534319 [Xylella fastidiosa subsp. morus]
MNNETFSRYDTADYLKTEEDIAAYMEAVMEEGGRDNPAFIARALGAVARARNLSQLARDVGMSRQGLDKALSIDGNPSFS
TILKVAKALGLRMSFTPSSMS

Nucleotide

• Length: 306 bp

>AT230426 NZ_CP124894:2727338-2727536 [Enterococcus faecalis]
TTTTTAGGGAAATGTGCTATAATGAAAACGAAAAGAGAGATATGCTTCAACATACCTCTCTGATGTAGAGCCGTTTAAGA
CGGTGACCGATTGTATTATTTAAAAATAACCGTACTGGTCAAAGTAGACGGTTATTTTTTATTGTCATTTTTAAGCAATT
TCACAATCAGTGCAATCAAAGCAATGGTAAACATACCAA

Structures

Toxin

• AlphaFold DB: A0A060HBK9