Detailed information of TA system TA230425

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 530707..531318
• Replicon: chromosome
• Accession: NZ_CP090316
• Organism: Xylella fastidiosa subsp. morus strain Riv16

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A060HHU8
• Locus tag: IUD23_RS02495
• Protein ID: WP_024749166.1
• Coordinates: 531019..531318 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: Q879U0
• Locus tag: IUD23_RS02490
• Protein ID: WP_004085003.1
• Coordinates: 530707..531015 (-)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IUD23_RS02465 (IUD23_02465)	525829..526233	+	405	WP_230577601.1	hypothetical protein	-
IUD23_RS02470 (IUD23_02470)	526234..527730	+	1497	WP_038230289.1	DUF3383 domain-containing protein	-
IUD23_RS02475 (IUD23_02475)	527740..528177	+	438	WP_038230291.1	DUF3277 family protein	-
IUD23_RS02480 (IUD23_02480)	528174..528596	+	423	WP_004085005.1	putative phage tail assembly chaperone	-
IUD23_RS02485 (IUD23_02485)	528744..530633	+	1890	WP_038230294.1	hypothetical protein	-
IUD23_RS02490 (IUD23_02490)	530707..531015	-	309	WP_004085003.1	putative addiction module antidote protein	Antitoxin
IUD23_RS02495 (IUD23_02495)	531019..531318	-	300	WP_024749166.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IUD23_RS02500 (IUD23_02500)	531406..531711	+	306	WP_230577600.1	hypothetical protein	-
IUD23_RS02505 (IUD23_02505)	531727..532176	+	450	WP_230577599.1	hypothetical protein	-
IUD23_RS02510 (IUD23_02510)	532176..532493	+	318	WP_012382585.1	hypothetical protein	-
IUD23_RS02515 (IUD23_02515)	532490..533320	+	831	WP_234056377.1	hypothetical protein	-
IUD23_RS02520 (IUD23_02520)	533317..533958	+	642	WP_040123250.1	Gp138 family membrane-puncturing spike protein	-
IUD23_RS02525 (IUD23_02525)	533955..534308	+	354	WP_234056392.1	hypothetical protein	-
IUD23_RS02530 (IUD23_02530)	534319..534624	-	306	WP_038211623.1	putative addiction module antidote protein	-
IUD23_RS02535 (IUD23_02535)	534628..534924	-	297	WP_004089252.1	type II toxin-antitoxin system RelE/ParE family toxin	-
IUD23_RS02540 (IUD23_02540)	535023..536168	+	1146	WP_038211620.1	baseplate J/gp47 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	422869..537892	115023

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-98)

Antitoxin

• Uncharacterized protein HI1420 (15-100)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11364.14 Da
• Isoelectric Point: 10.3861

>T230425 WP_024749166.1 NZ_CP090316:c531318-531019 [Xylella fastidiosa subsp. morus]
MTYTVKRLEGFSDWLKGLKDGLARQRLIKRLRKVQLGNFGDVQPVGEGVFEMREHFGPGWRMYYVQRGSFLIVMLGGGDK
STQQSDIRRAIELAKSLED

Nucleotide

• Length: 300 bp

>T230425 NZ_CP124894:c2727237-2727094 [Enterococcus faecalis]
ATGAGCGTACTTCCAAAATTTACAGAAAGGAGAGGCCTGTTGTCAGCATATGAAACAATTCAGACGATCCTAGGGTTTGG
TATGTTTACCATTGCTTTGATTGCGCTGATTGTGAAATTGCTTAAAAATGACAATAAAAAATAA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11060.61 Da
• Isoelectric Point: 4.6535

>AT230425 WP_004085003.1 NZ_CP090316:c531015-530707 [Xylella fastidiosa subsp. morus]
VTITKKINVSELPEFDAAEYLNSEEEVAAYLTAVLEENDPALLAAALGDIARSRGMSQIAKDSGITREALYKALRPGSEP
RFDTISRVCTALGIRLVAQPMH

Nucleotide

• Length: 309 bp

>AT230425 NZ_CP124894:2726975-2727161 [Enterococcus faecalis]
TGTGCTATAATGAAAACGAAAAGAGAGATATGCGTCAACATACCTCTCTGATGTAGAGCCGTTTAAGACGGTGACCGATT
TTGTTACAAAAAATAACCGTACTCGATCAAAGTAAACGGTTATTTTTTATTGTCATTTTTAAGCAATTTCACAATCAGCG
CAATCAAAGCAATGGTAAACATACCAA

Structures

Toxin

• AlphaFold DB: A0A060HHU8

Antitoxin

• AlphaFold DB: A0A060HCD4