Detailed information of TA system TA230422

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 479715..480326
• Replicon: chromosome
• Accession: NZ_CP090316
• Organism: Xylella fastidiosa subsp. morus strain Riv16

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A060HHU8
• Locus tag: IUD23_RS02120
• Protein ID: WP_024749166.1
• Coordinates: 480027..480326 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: Q879U0
• Locus tag: IUD23_RS02115
• Protein ID: WP_004085003.1
• Coordinates: 479715..480023 (-)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IUD23_RS02090 (IUD23_02090)	474838..475242	+	405	WP_230577601.1	hypothetical protein	-
IUD23_RS02095 (IUD23_02095)	475243..476738	+	1496	Protein_397	DUF3383 domain-containing protein	-
IUD23_RS02100 (IUD23_02100)	476748..477185	+	438	WP_038230291.1	DUF3277 family protein	-
IUD23_RS02105 (IUD23_02105)	477182..477604	+	423	WP_004085005.1	putative phage tail assembly chaperone	-
IUD23_RS02110 (IUD23_02110)	477752..479641	+	1890	WP_038230294.1	hypothetical protein	-
IUD23_RS02115 (IUD23_02115)	479715..480023	-	309	WP_004085003.1	putative addiction module antidote protein	Antitoxin
IUD23_RS02120 (IUD23_02120)	480027..480326	-	300	WP_024749166.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IUD23_RS02125 (IUD23_02125)	480414..480719	+	306	WP_230577600.1	hypothetical protein	-
IUD23_RS02130 (IUD23_02130)	480735..481184	+	450	WP_230577599.1	hypothetical protein	-
IUD23_RS02135 (IUD23_02135)	481184..481501	+	318	WP_012382585.1	hypothetical protein	-
IUD23_RS02140 (IUD23_02140)	481498..482328	+	831	WP_234056377.1	hypothetical protein	-
IUD23_RS02145 (IUD23_02145)	482325..482966	+	642	WP_040123250.1	Gp138 family membrane-puncturing spike protein	-
IUD23_RS02150 (IUD23_02150)	482963..483316	+	354	WP_040123277.1	hypothetical protein	-
IUD23_RS02155 (IUD23_02155)	483372..483665	-	294	WP_004085592.1	putative addiction module antidote protein	-
IUD23_RS02160 (IUD23_02160)	483668..483964	-	297	WP_004085590.1	type II toxin-antitoxin system RelE/ParE family toxin	-
IUD23_RS02165 (IUD23_02165)	484067..485212	+	1146	WP_038211620.1	baseplate J/gp47 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	422869..537892	115023

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-98)

Antitoxin

• Uncharacterized protein HI1420 (15-100)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11364.14 Da
• Isoelectric Point: 10.3861

>T230422 WP_024749166.1 NZ_CP090316:c480326-480027 [Xylella fastidiosa subsp. morus]
MTYTVKRLEGFSDWLKGLKDGLARQRLIKRLRKVQLGNFGDVQPVGEGVFEMREHFGPGWRMYYVQRGSFLIVMLGGGDK
STQQSDIRRAIELAKSLED

Nucleotide

• Length: 300 bp

>T230422 NZ_CP124894:567876-567992 [Enterococcus faecalis]
ATGTTTTTGTCCGTTGAAGCAGCGTTGGGACTGATGATTGGTTTTGCAACACTTGTTGTGACCATTATCTTCGGTATCTT
AGCGCTTGTCTTAGACAACAAAAATAACCGTTCATAA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11060.61 Da
• Isoelectric Point: 4.6535

>AT230422 WP_004085003.1 NZ_CP090316:c480023-479715 [Xylella fastidiosa subsp. morus]
VTITKKINVSELPEFDAAEYLNSEEEVAAYLTAVLEENDPALLAAALGDIARSRGMSQIAKDSGITREALYKALRPGSEP
RFDTISRVCTALGIRLVAQPMH

Nucleotide

• Length: 309 bp

>AT230422 NZ_CP124894:c568108-567903 [Enterococcus faecalis]
AAAAGAGAGATATGCAGGAACATACCTCTCTAGTAGCCACTACTAGTTATAAGAACTAGCGGCTTACTGAGTTATTGGTT
TTATTTCAAACGCTTAACCGTTCAGCTCCTCAAAGCTTATGAACGGTTATTTTTGTTGTCTAAGACAAGCGCTAAGATAC
CGAAGATAATGGTCACAACAAGTGTTGCAAAACCAATCATCAGTCC

Structures

Toxin

• AlphaFold DB: A0A060HHU8

Antitoxin

• AlphaFold DB: A0A060HCD4