Detailed information of TA system TA230359

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yeeU/CbtA-CbeA
• Location: 115041..115834
• Replicon: chromosome
• Accession: NZ_CP090245
• Organism: Klebsiella oxytoca strain KONSF-1

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: -
• Locus tag: LXH16_RS00485
• Protein ID: WP_234284000.1
• Coordinates: 115041..115430 (-)
• Length: 130 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: LXH16_RS00490
• Protein ID: WP_200493555.1
• Coordinates: 115469..115834 (-)
• Length: 122 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LXH16_RS00480 (LXH16_00480)	110259..113774	-	3516	WP_142448058.1	AAA domain-containing protein	-
LXH16_RS00485 (LXH16_00485)	115041..115430	-	390	WP_234284000.1	TA system toxin CbtA family protein	Toxin
LXH16_RS00490 (LXH16_00490)	115469..115834	-	366	WP_200493555.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
LXH16_RS00495 (LXH16_00495)	115860..116081	-	222	WP_200493554.1	DUF987 family protein	-
LXH16_RS00500 (LXH16_00500)	116078..116620	-	543	WP_234284001.1	DNA repair protein RadC	-
LXH16_RS00505 (LXH16_00505)	116633..117076	-	444	WP_200493552.1	antirestriction protein	-
LXH16_RS00510 (LXH16_00510)	117107..117928	-	822	WP_200493551.1	DUF932 domain-containing protein	-
LXH16_RS00515 (LXH16_00515)	118027..118251	-	225	WP_200493550.1	DUF905 domain-containing protein	-
LXH16_RS00520 (LXH16_00520)	118323..118772	-	450	WP_200493549.1	IrmA family protein	-
LXH16_RS00525 (LXH16_00525)	118769..119221	-	453	WP_200493548.1	hypothetical protein	-
LXH16_RS00530 (LXH16_00530)	119258..119827	-	570	WP_200493559.1	hypothetical protein	-
LXH16_RS00535 (LXH16_00535)	119827..120531	-	705	WP_200493547.1	WYL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	105610..146710	41100

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (4-112)

Antitoxin

• Antitoxin CbeA (17-117)

Sequences

Toxin

Protein

• Length: 130 a.a.
• Molecular weight: 14549.65 Da
• Isoelectric Point: 9.1643

>T230359 WP_234284000.1 NZ_CP090245:c115430-115041 [Klebsiella oxytoca]
MQKISSHPIRANQPCLSPIEIWQRLLTHLLSQHYGLTLNDTPFSNDSTIQEHIDAGVSLSDAVNFLVEKYELERIDRDGC
TVMEKSPFITSIDILRARKASGLMKRSSHKTVTRVTAGHHQQLKITKGT

Nucleotide

• Length: 390 bp

>T230359 NZ_CP124884:c317838-317743 [Enterococcus faecalis]
ATGTACGAGATTGTCACAAAAATTCTTGTGCCTATTTTTGTCGGGATTGTCCTGAAACTTGTAACCATTTGGTTGGAAAA
ACAGAACGAGGAATAA

Antitoxin

Protein

• Length: 122 a.a.
• Molecular weight: 13561.21 Da
• Isoelectric Point: 4.5927

>AT230359 WP_200493555.1 NZ_CP090245:c115834-115469 [Klebsiella oxytoca]
MNNHSESGTTPENPTCQQWGLKSTITPCFGARLVQEDCRIHFLADRAGFNGAFSDNDTLRLDQAFPLMLKQLELMLTSGE
LNPRYQHCVTLYHNGLTCEADTLGSCGYVYIAIYPEQTEPQ

Nucleotide

• Length: 366 bp

>AT230359 NZ_CP124884:317643-317708 [Enterococcus faecalis]
CTTGTGACAAAGTCGTGCATGGATGCACTAAAAAGACACCCTATCAGTAGTAGGGTGTCTTTTTTT