Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /RES-TIGR02293
Location 136572..137482 Replicon plasmid pKPNSF-2-1
Accession NZ_CP090243
Organism Klebsiella pneumoniae strain KPNSF-2

Toxin (Protein)


Gene name - Uniprot ID A0A663AYG0
Locus tag LXH14_RS26965 Protein ID WP_004026354.1
Coordinates 137012..137482 (+) Length 157 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A6P1V3Q9
Locus tag LXH14_RS26960 Protein ID WP_004026357.1
Coordinates 136572..137015 (+) Length 148 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LXH14_RS26930 (LXH14_26930) 131590..132111 + 522 WP_004213635.1 ferric citrate uptake sigma factor FecI -
LXH14_RS26935 (LXH14_26935) 132108..133061 + 954 Protein_146 fec operon regulator FecR -
LXH14_RS26940 (LXH14_26940) 133148..135274 + 2127 WP_004213640.1 TonB-dependent Fe(3+) dicitrate receptor FecA -
LXH14_RS26945 (LXH14_26945) 135349..135468 + 120 Protein_148 Fe3+-citrate ABC transporter substrate-binding protein -
LXH14_RS26950 (LXH14_26950) 135649..135870 + 222 WP_004213643.1 DUF2188 domain-containing protein -
LXH14_RS26955 (LXH14_26955) 135968..136471 + 504 Protein_150 DUF4113 domain-containing protein -
LXH14_RS26960 (LXH14_26960) 136572..137015 + 444 WP_004026357.1 DUF2384 domain-containing protein Antitoxin
LXH14_RS26965 (LXH14_26965) 137012..137482 + 471 WP_004026354.1 RES family NAD+ phosphorylase Toxin
LXH14_RS26970 (LXH14_26970) 137631..138328 + 698 WP_223175001.1 IS1-like element IS1A family transposase -
LXH14_RS26975 (LXH14_26975) 138330..138779 + 450 WP_011251283.1 thioredoxin fold domain-containing protein -
LXH14_RS26980 (LXH14_26980) 138796..139107 + 312 WP_011251282.1 hypothetical protein -
LXH14_RS26985 (LXH14_26985) 139121..139462 + 342 WP_011251281.1 hypothetical protein -
LXH14_RS26990 (LXH14_26990) 139522..140478 + 957 WP_011251280.1 DsbA family protein -
LXH14_RS26995 (LXH14_26995) 140595..141170 + 576 WP_074424927.1 hypothetical protein -
LXH14_RS27000 (LXH14_27000) 141278..141793 + 516 WP_041937820.1 hypothetical protein -
LXH14_RS27005 (LXH14_27005) 141796..142398 + 603 WP_011251277.1 HAD domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - rmpA2 / iutA / iucD / iucC / iucB / iucA / rmpA / iroN / iroD / iroC / iroB 1..226910 226910
- flank IS/Tn - - 137825..138328 503


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(3-148)

Antitoxin

Antitoxin Xre-like, helix-turn-helix domain

(30-89)


Sequences

Toxin        


Download         Length: 157 a.a.        Molecular weight: 17515.79 Da        Isoelectric Point: 4.6155

>T230339 WP_004026354.1 NZ_CP090243:137012-137482 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Download         Length: 471 bp

>T230339 NZ_CP124873:1924721-1924823 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 148 a.a.        Molecular weight: 16480.80 Da        Isoelectric Point: 10.2498

>AT230339 WP_004026357.1 NZ_CP090243:136572-137015 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDKEAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Download         Length: 444 bp

>AT230339 NZ_CP124873:c1924830-1924685 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A663AYG0

Antitoxin

Source ID Structure
AlphaFold DB A0A6P1V3Q9

References