Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 3677270..3677895 Replicon chromosome
Accession NZ_CP090242
Organism Klebsiella pneumoniae strain KPNSF-2

Toxin (Protein)


Gene name vapC Uniprot ID A0A330XB05
Locus tag LXH14_RS17560 Protein ID WP_012737091.1
Coordinates 3677270..3677653 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag LXH14_RS17565 Protein ID WP_004150355.1
Coordinates 3677653..3677895 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LXH14_RS17545 (3674636) 3674636..3675538 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
LXH14_RS17550 (3675535) 3675535..3676170 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
LXH14_RS17555 (3676167) 3676167..3677096 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
LXH14_RS17560 (3677270) 3677270..3677653 - 384 WP_012737091.1 type II toxin-antitoxin system VapC family toxin Toxin
LXH14_RS17565 (3677653) 3677653..3677895 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
LXH14_RS17570 (3678100) 3678100..3679017 + 918 WP_012737090.1 alpha/beta hydrolase -
LXH14_RS17575 (3679031) 3679031..3679972 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
LXH14_RS17580 (3680017) 3680017..3680454 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
LXH14_RS17585 (3680451) 3680451..3681311 - 861 WP_004146232.1 virulence factor BrkB family protein -
LXH14_RS17590 (3681305) 3681305..3681904 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-110)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14266.53 Da        Isoelectric Point: 7.2771

>T230334 WP_012737091.1 NZ_CP090242:c3677653-3677270 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRCEAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKGFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T230334 NZ_CP124870:28354-28605 [Klebsiella quasipneumoniae]
ATGAGAAAAAATAAAGGTGATGTGACTTATTTTCTTGAAAAAGAAGGTGACAATTACAGGCTTACAAAAAGGATAAAAGC
CAGAACAAATGTTAAAATCGGGAACAAAACTACGAAAATTACATTGTATGATGCTGTACTGAATGAGAATGAATTACAAC
ATATTGATTTTACGTGTGCCGGATTAAGGGAAGACGATGAGACGCCAGTTAAGAATTTAATTAAGGAGTTTATGTTAAAT
GAAACCCGATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT230334 WP_004150355.1 NZ_CP090242:c3677895-3677653 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT230334 NZ_CP124870:28592-28819 [Klebsiella quasipneumoniae]
ATGAAACCCGATAATACACCTGAAAATGTAAAAAAATTACGTTTAAAAGCAGGATTAACACAAAAGGAATGCAGTCATAT
TTATGGTGTTGGGCTAAGAACCTGGCAGAAAAAAGAGGAAGTTAATACTCAAAACAGTCAAAGTCTGTCACTGGTTGAGT
TTGAGTTTCTTTTGTTACTTGCAGGAGAACATCCTGAATACGTGCTCTGCAAAAGGGAGTCAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A330XB05

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References