Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 3039053..3039863 Replicon chromosome
Accession NZ_CP090242
Organism Klebsiella pneumoniae strain KPNSF-2

Toxin (Protein)


Gene name KacT Uniprot ID A0A332NV98
Locus tag LXH14_RS14585 Protein ID WP_014908042.1
Coordinates 3039053..3039586 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag LXH14_RS14590 Protein ID WP_002887278.1
Coordinates 3039597..3039863 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LXH14_RS14580 (3037884) 3037884..3039005 + 1122 WP_012737335.1 cupin domain-containing protein -
LXH14_RS14585 (3039053) 3039053..3039586 - 534 WP_014908042.1 type II toxin-antitoxin system toxin KacT Toxin
LXH14_RS14590 (3039597) 3039597..3039863 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
LXH14_RS14595 (3039966) 3039966..3041399 - 1434 WP_014908043.1 Cu(+)/Ag(+) sensor histidine kinase -
LXH14_RS14600 (3041389) 3041389..3042072 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
LXH14_RS14605 (3042244) 3042244..3043629 + 1386 WP_012737332.1 efflux transporter outer membrane subunit -
LXH14_RS14610 (3043647) 3043647..3043991 + 345 WP_012737331.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(87-150)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19795.68 Da        Isoelectric Point: 5.2614

>T230332 WP_014908042.1 NZ_CP090242:c3039586-3039053 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGLGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T230332 NZ_CP124866:c4573932-4573648 [Klebsiella quasipneumoniae]
ATGACCTATGAACTGGCGTTTGATCCGCGTGCCTGGCGCGAATGGCAGAAGCTTGGCGAGACGATCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGGTTGTGCAGAATCCGCGAACTGAGTCGGCCAGGCTGAGCGATTTGCCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGCTGGTGTATCAAGTACGAGATAGGGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT230332 WP_002887278.1 NZ_CP090242:c3039863-3039597 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT230332 NZ_CP124866:c4574164-4573922 [Klebsiella quasipneumoniae]
ATGGCCACGCTCAACGTTCGACTGGATGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACGCC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACTATCAGCGATA
GCGAAGCGGCATTATTACGCACCGTCCGCGAGCGGCTGGAAAACCCGCAGCCGGAGGTGAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A332NV98

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References