Detailed information of TA system TA023032

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF-MazE
• Location: 31125..31696
• Replicon: chromosome
• Accession: NC_011146
• Organism: Geobacter bemidjiensis Bem

Toxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: GBEM_RS00120
• Protein ID: WP_041262458.1
• Coordinates: 31125..31454 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazF
• Uniprot ID: B5EJ30
• Locus tag: GBEM_RS00125
• Protein ID: WP_012528466.1
• Coordinates: 31442..31696 (-)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GBEM_RS00095	26173..26454	+	282	WP_012528460.1	GIY-YIG nuclease family protein	-
GBEM_RS00100	26479..27516	-	1038	WP_012528461.1	cytochrome-c peroxidase	-
GBEM_RS00105	27683..29092	-	1410	WP_012528462.1	NADPH-dependent glutamate synthase	-
GBEM_RS00110	29089..29937	-	849	WP_012528463.1	sulfide/dihydroorotate dehydrogenase-like FAD/NAD-binding protein	-
GBEM_RS00115	30217..31092	-	876	WP_012528464.1	DMT family transporter	-
GBEM_RS00120	31125..31454	-	330	WP_041262458.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
GBEM_RS00125	31442..31696	-	255	WP_012528466.1	antitoxin MazE	Antitoxin
GBEM_RS00135	31964..32806	-	843	WP_012528467.1	FAD/NAD(P)-binding protein	-
GBEM_RS00140	32984..33994	-	1011	WP_012528468.1	4Fe-4S dicluster domain-containing protein	-
GBEM_RS00145	34153..35097	-	945	WP_012528469.1	4Fe-4S dicluster domain-containing protein	-
GBEM_RS00150	35094..35621	-	528	WP_012528470.1	hydrogenase iron-sulfur subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (6-107)

Antitoxin

• SpoVT-AbrB domain (7-46)

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 12248.63 Da
• Isoelectric Point: 10.4290

>T23032 WP_041262458.1 NC_011146:c31454-31125 [Geobacter bemidjiensis Bem]
MGMVVKRFDIFMVNLDPTVGSEIRKFRPCLIISPDEMNENIATVIVAPMTTKGRGYPTRVPCTFKGKDGQVILDQIRTVD
KSRLIHKQGKLAKDVQEKVLSILAKMFAL

Nucleotide

• Length: 330 bp

>T23032 NC_011146:c31454-31125 [Geobacter bemidjiensis Bem]
ATGGGAATGGTAGTTAAACGTTTCGACATCTTCATGGTCAATCTTGACCCTACGGTTGGCAGCGAGATTCGCAAATTCCG
CCCGTGCCTCATTATTTCTCCCGACGAGATGAACGAAAATATTGCCACTGTCATCGTAGCGCCGATGACAACAAAGGGCA
GAGGTTACCCGACGCGGGTTCCCTGCACATTCAAGGGAAAGGATGGGCAGGTCATCCTCGATCAGATACGAACGGTAGAC
AAGTCTCGCCTCATTCATAAGCAAGGCAAACTCGCCAAAGACGTCCAGGAAAAAGTTTTGTCCATTCTGGCTAAAATGTT
TGCTCTATAA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9850.03 Da
• Isoelectric Point: 4.0953

>AT23032 WP_012528466.1 NC_011146:c31696-31442 [Geobacter bemidjiensis Bem]
MKTNIIRIGNSQGVRIPKILLEQSRLGTEVELEVQNEMIVIRSATQPRDGWAEQFRLMSECGDDKMIDEDPGEQTDWDKE
EWEW

Nucleotide

• Length: 255 bp

>AT23032 NC_011146:c31696-31442 [Geobacter bemidjiensis Bem]
ATGAAGACCAATATTATTCGCATCGGTAATTCACAAGGGGTACGTATTCCGAAAATCCTCCTTGAACAGAGTCGTCTCGG
AACTGAGGTGGAATTGGAAGTACAGAACGAAATGATCGTGATACGGTCGGCCACTCAACCTCGAGACGGGTGGGCTGAAC
AATTCAGGCTTATGTCCGAGTGCGGCGACGACAAGATGATTGACGAGGACCCGGGCGAACAAACGGACTGGGACAAGGAA
GAATGGGAATGGTAG

Structures

Antitoxin

• AlphaFold DB: B5EJ30