Detailed information of TA system TA230303

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 54500..55236
• Replicon: plasmid pBY221-2
• Accession: NZ_CP090220
• Organism: Klebsiella pneumoniae strain BY221

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: L7SZ15
• Locus tag: LVJ43_RS26810
• Protein ID: WP_003026803.1
• Coordinates: 54754..55236 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: LVJ43_RS26805
• Protein ID: WP_003026799.1
• Coordinates: 54500..54766 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ43_RS26765 (LVJ43_26740)	49729..50091	-	363	WP_004152100.1	arsenite efflux transporter metallochaperone ArsD	-
LVJ43_RS26770 (LVJ43_26745)	50141..50491	-	351	WP_004152101.1	As(III)-sensing metalloregulatory transcriptional repressor ArsR	-
LVJ43_RS26775 (LVJ43_26750)	50849..51097	+	249	WP_001568018.1	hypothetical protein	-
LVJ43_RS26780 (LVJ43_26755)	51094..51666	+	573	WP_001568017.1	hypothetical protein	-
LVJ43_RS26785 (LVJ43_26760)	51697..52191	+	495	WP_001568016.1	hypothetical protein	-
LVJ43_RS26790 (LVJ43_26765)	52424..52774	-	351	WP_167878626.1	hypothetical protein	-
LVJ43_RS26795 (LVJ43_26770)	53226..53774	+	549	WP_001567366.1	thioredoxin fold domain-containing protein	-
LVJ43_RS26800 (LVJ43_26775)	53821..54255	+	435	WP_001567367.1	cell envelope integrity protein TolA	-
LVJ43_RS26805 (LVJ43_26780)	54500..54766	+	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
LVJ43_RS26810 (LVJ43_26785)	54754..55236	+	483	WP_003026803.1	GNAT family N-acetyltransferase	Toxin
LVJ43_RS26815 (LVJ43_26790)	55437..56827	+	1391	Protein_56	ISNCY-like element ISKpn21 family transposase	-
LVJ43_RS26820 (LVJ43_26795)	56856..57488	-	633	WP_001567369.1	hypothetical protein	-
LVJ43_RS26825 (LVJ43_26800)	57714..59060	+	1347	WP_077267029.1	ISNCY family transposase	-
LVJ43_RS26830 (LVJ43_26805)	59109..59513	+	405	WP_064145647.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aac(3)-IId / aph(3')-Ia	-	1..193044	193044

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17353.97 Da
• Isoelectric Point: 9.5822

>T230303 WP_003026803.1 NZ_CP090220:54754-55236 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T230303 NZ_CP124837:327498-327866 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCAGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACTCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCGGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCGGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGCTTGCGCGGATGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT230303 WP_003026799.1 NZ_CP090220:54500-54766 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT230303 NZ_CP124837:327280-327501 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGACGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Toxin

• AlphaFold DB: A0A0J2GNW6

Antitoxin

• AlphaFold DB: A0A1Q8YL66