Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 94621..95264 Replicon plasmid pBY221-1
Accession NZ_CP090219
Organism Klebsiella pneumoniae strain BY221

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag LVJ43_RS25985 Protein ID WP_001044770.1
Coordinates 94621..95037 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag LVJ43_RS25990 Protein ID WP_001261282.1
Coordinates 95034..95264 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LVJ43_RS25965 (90724) 90724..90996 - 273 Protein_113 transposase -
LVJ43_RS25975 (91978) 91978..93000 - 1023 WP_000361404.1 helicase UvrD -
LVJ43_RS25980 (92985) 92985..94547 - 1563 WP_004206609.1 AAA family ATPase -
LVJ43_RS25985 (94621) 94621..95037 - 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
LVJ43_RS25990 (95034) 95034..95264 - 231 WP_001261282.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
LVJ43_RS25995 (95221) 95221..95682 + 462 WP_014343465.1 hypothetical protein -
LVJ43_RS26000 (95843) 95843..96787 + 945 WP_011977810.1 hypothetical protein -
LVJ43_RS26005 (96824) 96824..97216 + 393 WP_011977811.1 hypothetical protein -
LVJ43_RS26010 (97274) 97274..97795 + 522 WP_094960442.1 hypothetical protein -
LVJ43_RS26015 (97841) 97841..98044 + 204 WP_011977813.1 hypothetical protein -
LVJ43_RS26020 (98074) 98074..99078 + 1005 WP_011977814.1 hypothetical protein -
LVJ43_RS26025 (99262) 99262..100041 + 780 WP_013214009.1 site-specific integrase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaCTX-M-65 / fosA3 / blaTEM-1B / rmtB / blaKPC-2 / catA2 - 1..172001 172001


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T230301 WP_001044770.1 NZ_CP090219:c95037-94621 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T230301 NZ_CP124828:53921-54403 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT230301 WP_001261282.1 NZ_CP090219:c95264-95034 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT230301 NZ_CP124828:53667-53933 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References