Detailed information of TA system TA230276

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Xre-MNT/HTH_26(antitoxin)
• Location: 1870516..1871624
• Replicon: chromosome
• Accession: NZ_CP090216
• Organism: Vagococcus lutrae strain VF20

Toxin (Protein)

• Gene name: MNTss
• Uniprot ID: R3JIN1
• Locus tag: LVJ09_RS08990
• Protein ID: WP_000233000.1
• Coordinates: 1870516..1871385 (-)
• Length: 290 a.a.

Antitoxin (Protein)

• Gene name: Xress
• Uniprot ID: -
• Locus tag: LVJ09_RS08995
• Protein ID: WP_000205227.1
• Coordinates: 1871400..1871624 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LVJ09_RS08970 (LVJ09_08960)	1867581..1868261	+	681	WP_001015311.1	IS6-like element IS1216 family transposase	-
LVJ09_RS08975 (LVJ09_08965)	1868334..1868861	-	528	WP_002294507.1	phosphoribosyltransferase family protein	-
LVJ09_RS08980 (LVJ09_08970)	1868905..1869768	-	864	WP_002294505.1	aminoglycoside nucleotidyltransferase ANT(6)-Ia	-
LVJ09_RS08985 (LVJ09_08975)	1869801..1870535	-	735	WP_000662263.1	class I SAM-dependent methyltransferase	-
LVJ09_RS08990 (LVJ09_08980)	1870516..1871385	-	870	WP_000233000.1	nucleotidyltransferase domain-containing protein	Toxin
LVJ09_RS08995 (LVJ09_08985)	1871400..1871624	-	225	WP_000205227.1	helix-turn-helix transcriptional regulator	Antitoxin
LVJ09_RS09000 (LVJ09_08990)	1871767..1871853	-	87	Protein_1737	site-specific recombinase	-
LVJ09_RS09005 (LVJ09_08995)	1872062..1873344	+	1283	Protein_1738	ISL3 family transposase	-
LVJ09_RS09010 (LVJ09_09000)	1873653..1874456	-	804	WP_002294514.1	lincosamide nucleotidyltransferase Lnu(B)	-
LVJ09_RS09015 (LVJ09_09005)	1874510..1875994	-	1485	WP_002294513.1	ABC-F type ribosomal protection protein Lsa(E)	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polymerase, nucleotidyl transferase domain (12-111)

Antitoxin

• Cro/C1-type helix-turn-helix domain (4-57)

Sequences

Toxin

Protein

• Length: 290 a.a.
• Molecular weight: 32926.57 Da
• Isoelectric Point: 4.8781

>T230276 WP_000233000.1 NZ_CP090216:c1871385-1870516 [Vagococcus lutrae]
MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIYYNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDW
INGGGWLFINGYHVDLILRDIKRVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYCINEKKAIKLLETFEYKPEKYAE
RVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINNFLNEESSDERKQI

Nucleotide

• Length: 870 bp

>T230276 NZ_CP124824:733586-734071 [Klebsiella pneumoniae]
ATGATCTCGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGACCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCGCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATTCGATGATGTTGATGGTGACGTTGAGGGATTTGGTTAAT
GCCTGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8365.68 Da
• Isoelectric Point: 4.5510

>AT230276 WP_000205227.1 NZ_CP090216:c1871624-1871400 [Vagococcus lutrae]
MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGEEIKISNE

Nucleotide

• Length: 225 bp

>AT230276 NZ_CP124824:733296-733589 [Klebsiella pneumoniae]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA

Structures

Toxin

• AlphaFold DB: R3JIN1