Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | vagCD/VapC-VagC |
| Location | 84533..85176 | Replicon | plasmid pBY68-1 |
| Accession | NZ_CP090213 | ||
| Organism | Escherichia coli strain BY68 | ||
Toxin (Protein)
| Gene name | vagD | Uniprot ID | Q84A06 |
| Locus tag | LVJ26_RS23770 | Protein ID | WP_000754566.1 |
| Coordinates | 84760..85176 (+) | Length | 139 a.a. |
Antitoxin (Protein)
| Gene name | vagC | Uniprot ID | Q84A07 |
| Locus tag | LVJ26_RS23765 | Protein ID | WP_001261276.1 |
| Coordinates | 84533..84763 (+) | Length | 77 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| LVJ26_RS23730 (LVJ26_23650) | 79683..80573 | + | 891 | Protein_88 | IS30-like element IS30 family transposase | - |
| LVJ26_RS23735 (LVJ26_23655) | 80596..81624 | - | 1029 | WP_063097364.1 | tyrosine-type recombinase/integrase | - |
| LVJ26_RS23740 (LVJ26_23660) | 82018..82493 | + | 476 | Protein_90 | IS1 family transposase | - |
| LVJ26_RS23745 (LVJ26_23665) | 82770..82979 | - | 210 | Protein_91 | IS110 family transposase | - |
| LVJ26_RS23750 (LVJ26_23670) | 83033..83737 | + | 705 | WP_001067855.1 | IS6-like element IS26 family transposase | - |
| LVJ26_RS23755 (LVJ26_23675) | 83843..84334 | + | 492 | Protein_93 | IS5-like element ISKpn26 family transposase | - |
| LVJ26_RS23760 (LVJ26_23680) | 84322..84576 | - | 255 | Protein_94 | hypothetical protein | - |
| LVJ26_RS23765 (LVJ26_23685) | 84533..84763 | + | 231 | WP_001261276.1 | type II toxin-antitoxin system VapB family antitoxin | Antitoxin |
| LVJ26_RS23770 (LVJ26_23690) | 84760..85176 | + | 417 | WP_000754566.1 | type II toxin-antitoxin system VapC family toxin | Toxin |
| LVJ26_RS23775 (LVJ26_23695) | 85381..86237 | + | 857 | Protein_97 | IS3 family transposase | - |
| LVJ26_RS23780 (LVJ26_23700) | 86809..87210 | + | 402 | WP_096171525.1 | hypothetical protein | - |
| LVJ26_RS23785 (LVJ26_23705) | 87310..87867 | - | 558 | WP_001235700.1 | recombinase family protein | - |
| LVJ26_RS23790 (LVJ26_23710) | 88031..88171 | + | 141 | WP_020219413.1 | hypothetical protein | - |
| LVJ26_RS23795 (LVJ26_23715) | 88183..88887 | + | 705 | WP_001067858.1 | IS6-like element IS26 family transposase | - |
| LVJ26_RS23800 (LVJ26_23720) | 88957..89157 | + | 201 | WP_000993264.1 | DUF3330 domain-containing protein | - |
| LVJ26_RS23805 (LVJ26_23725) | 89283..89843 | + | 561 | WP_000147567.1 | recombinase family protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Mobilizable plasmid | floR / aac(3)-IId / tet(M) / blaCARB-2 / aadA2 / cmlA1 / ant(3'')-Ia / sul3 / aph(3')-Ia | - | 1..103545 | 103545 | |
| - | inside | IScluster/Tn | - | - | 76590..99573 | 22983 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 139 a.a. Molecular weight: 15006.52 Da Isoelectric Point: 9.2957
>T230272 WP_000754566.1 NZ_CP090213:84760-85176 [Escherichia coli]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAVLPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNVREFARVPGLVLEDWVK
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAVLPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNVREFARVPGLVLEDWVK
Download Length: 417 bp
>T230272 NZ_CP124820:c42830-42543 [Escherichia coli]
ATGAGGTATCAGGTAAAATTCAGGGAAGATGCGCTGAAAGAGTGGCAAAAACTGGACAAGACTATTCAGCAACAGTTTGC
GAAAAAGCTAAAAAAGTGCTGTGACAATCCGCATATTCCTTCCGCAAAACTGCGCGGGATAAAGGACTGCTACAAAATAA
AATTACGTGCGTCAGGTTTTCGCCTAGTCTATCAGGTGATTGACGAACAATTAATTATCGCTGTTGTAGCTGTGGGTAAG
CGTGAGCGCAGTGACGTTTATAATCTTGCCAGTGAAAGAATGAGATAA
ATGAGGTATCAGGTAAAATTCAGGGAAGATGCGCTGAAAGAGTGGCAAAAACTGGACAAGACTATTCAGCAACAGTTTGC
GAAAAAGCTAAAAAAGTGCTGTGACAATCCGCATATTCCTTCCGCAAAACTGCGCGGGATAAAGGACTGCTACAAAATAA
AATTACGTGCGTCAGGTTTTCGCCTAGTCTATCAGGTGATTGACGAACAATTAATTATCGCTGTTGTAGCTGTGGGTAAG
CGTGAGCGCAGTGACGTTTATAATCTTGCCAGTGAAAGAATGAGATAA
Antitoxin
Download Length: 77 a.a. Molecular weight: 8721.74 Da Isoelectric Point: 4.3533
>AT230272 WP_001261276.1 NZ_CP090213:84533-84763 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP
Download Length: 231 bp
>AT230272 NZ_CP124820:c43078-42827 [Escherichia coli]
ATGCCTAACATTATTCTCAGTGATACAAGCGCCAGTGTCAGCGAGCTGAAGAAAAATCCGATGGCGACAGTCAGCGCCGG
TGATGGTTTCCCGATCGCTATCCTGAACCGTAATCAGCCTGCTTTCTACTGTGTACCCGCAGAGCTGTACGAAAAGATGC
TCGATGCCCTGGACGATCAGGAGCTGGTTAAACTGGTAGCCGAACGCAGCAACCAACCGCTGCATGATGTAGACCTAGAT
AAATACCTATGA
ATGCCTAACATTATTCTCAGTGATACAAGCGCCAGTGTCAGCGAGCTGAAGAAAAATCCGATGGCGACAGTCAGCGCCGG
TGATGGTTTCCCGATCGCTATCCTGAACCGTAATCAGCCTGCTTTCTACTGTGTACCCGCAGAGCTGTACGAAAAGATGC
TCGATGCCCTGGACGATCAGGAGCTGGTTAAACTGGTAGCCGAACGCAGCAACCAACCGCTGCATGATGTAGACCTAGAT
AAATACCTATGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A387K1G3 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A387K023 |