Detailed information of TA system TA230243

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 67462..68372
• Replicon: plasmid pJNKPN26_Vir
• Accession: NZ_CP090206
• Organism: Klebsiella pneumoniae strain JNKPN26

Toxin (Protein)

• Gene name: -
• Uniprot ID: A0A663AYG0
• Locus tag: LXM99_RS29125
• Protein ID: WP_004026354.1
• Coordinates: 67462..67932 (-)
• Length: 157 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A6P1V3Q9
• Locus tag: LXM99_RS29130
• Protein ID: WP_004026357.1
• Coordinates: 67929..68372 (-)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LXM99_RS29100 (LXM99_29100)	62874..63272	-	399	WP_004212796.1	H-NS family nucleoid-associated regulatory protein	-
LXM99_RS29105 (LXM99_29105)	63609..64103	-	495	WP_004212794.1	thermonuclease family protein	-
LXM99_RS29110 (LXM99_29110)	64281..64547	+	267	WP_223175074.1	DUF1173 family protein	-
LXM99_RS29115 (LXM99_29115)	64580..65230	+	651	WP_068893702.1	DUF1173 family protein	-
LXM99_RS29125 (LXM99_29125)	67462..67932	-	471	WP_004026354.1	RES family NAD+ phosphorylase	Toxin
LXM99_RS29130 (LXM99_29130)	67929..68372	-	444	WP_004026357.1	DUF2384 domain-containing protein	Antitoxin
LXM99_RS29135 (LXM99_29135)	68473..68976	-	504	Protein_76	DUF4113 domain-containing protein	-
LXM99_RS29140 (LXM99_29140)	69182..70162	-	981	WP_000019473.1	IS5-like element ISKpn26 family transposase	-
LXM99_RS29145 (LXM99_29145)	70228..71535	+	1308	Protein_78	FepA family TonB-dependent siderophore receptor	-
LXM99_RS29150 (LXM99_29150)	71597..71818	+	222	WP_004213615.1	hypothetical protein	-
LXM99_RS29155 (LXM99_29155)	71961..72863	-	903	WP_004213613.1	DMT family inner membrane transporter PEG344	-
LXM99_RS29160 (LXM99_29160)	72936..73082	-	147	Protein_81	DNA-binding response regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA2	1..220975	220975
	-	inside	IScluster/Tn	-	iroN / rmpA	61423..81157	19734

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre-like, helix-turn-helix domain (30-89)

Sequences

Toxin

Protein

• Length: 157 a.a.
• Molecular weight: 17515.79 Da
• Isoelectric Point: 4.6155

>T230243 WP_004026354.1 NZ_CP090206:c67932-67462 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Nucleotide

• Length: 471 bp

>T230243 NZ_CP124813:567283-567567 [Citrobacter freundii]
ATGACTTATGAACTGGAATTCGACCCGAGAGCCTTAAAAGAGTGGCATAAACTGGGTGATACGGTGAAGGCTCAGTTTAA
GAAGAAGTTGGCCGACGTGTTGTTGAACCCCAGAATTGACTCTGCCCGTTTAAACGGTCTTCCAGATTGCTATAAAATTA
AGCTTAAATCGTCCGGCTATCGTCTGGTGTACCAGGTTCGGGATGAGGTTGTGACCGTGTTTGTTGTCGCGGTCGGCAAG
CGACAACATTCGGCCGTTTATCTCGATGCAAACAAACGGCTTTAG

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16480.80 Da
• Isoelectric Point: 10.2498

>AT230243 WP_004026357.1 NZ_CP090206:c68372-67929 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDKEAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Nucleotide

• Length: 444 bp

>AT230243 NZ_CP124813:567051-567293 [Citrobacter freundii]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAATGAGGCTTATGCCGTGCTGGAAAAACTGAACATTACTCC
AACGGAAGCTGTGCGATTACTGTTTCAGTACGTCGCGGAAACGGGGCGCATGCCAGTGAAAACCGTCACCGTTAGCGACA
GCGAAGATACACTGATTCAGACAGTCCGGGAGAGGCTGGCCAGCCCGCAAAAAGGAATCAAGGTTAGTCTGGATGACTTA
TGA

Structures

Toxin

• AlphaFold DB: A0A663AYG0

Antitoxin

• AlphaFold DB: A0A6P1V3Q9