Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 98471..99114 Replicon plasmid pJNKPN26_KPC
Accession NZ_CP090204
Organism Klebsiella pneumoniae strain JNKPN26

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag LXM99_RS27760 Protein ID WP_001044770.1
Coordinates 98698..99114 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag LXM99_RS27755 Protein ID WP_001261282.1
Coordinates 98471..98701 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LXM99_RS27720 (93694) 93694..94473 - 780 WP_013214009.1 site-specific integrase -
LXM99_RS27725 (94657) 94657..95661 - 1005 WP_011977814.1 hypothetical protein -
LXM99_RS27730 (95691) 95691..95894 - 204 WP_011977813.1 hypothetical protein -
LXM99_RS27735 (95940) 95940..96461 - 522 WP_013214008.1 hypothetical protein -
LXM99_RS27740 (96519) 96519..96911 - 393 WP_011977811.1 hypothetical protein -
LXM99_RS27745 (96948) 96948..97892 - 945 WP_011977810.1 hypothetical protein -
LXM99_RS27750 (98053) 98053..98514 - 462 WP_014343465.1 hypothetical protein -
LXM99_RS27755 (98471) 98471..98701 + 231 WP_001261282.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
LXM99_RS27760 (98698) 98698..99114 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
LXM99_RS27765 (99188) 99188..100750 + 1563 WP_004206609.1 AAA family ATPase -
LXM99_RS27770 (100735) 100735..101757 + 1023 WP_000361404.1 helicase UvrD -
LXM99_RS27775 (102013) 102013..102710 + 698 WP_223174630.1 IS1 family transposase -
LXM99_RS27780 (102739) 102739..103011 + 273 Protein_146 transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid rmtB / blaTEM-1B / blaCTX-M-65 / blaKPC-2 / blaSHV-12 - 1..126203 126203


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T230240 WP_001044770.1 NZ_CP090204:98698-99114 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T230240 NZ_CP124813:34610-34972 [Citrobacter freundii]
ATGTGGGAAGTGGAAACAACGGATGCTTTCGATAAATGGTTTGACGTACAGACCGAAGCGCTCAAGGAAGATATGCTCGC
AGCAATGATGATTTTATCCGAGTATGGTCCTCAGTTAGGCAGGCCGTTTGCTGATACGGTAAATGCTTCTGCTTTTTCAA
ATATGAAAGAGTTGCGAGTACAGCATCAGGGTAGTCCGATTCGGGCGTTCTTCGCGTTTGATCCTTCTCGCCATGGCATC
GTGCTATGCGCTGGAGATAAAACAGGGTTAAACGAAAAGAAATTCTATAAAGAGATGATCAGGCTTGCGGATGCTGAGTA
CCGAAATCACCTAATAAGTAAGGAAAATTATGGCTACCCTTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT230240 WP_001261282.1 NZ_CP090204:98471-98701 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT230240 NZ_CP124813:34959-35258 [Citrobacter freundii]
ATGGCTACCCTTAAAGAATTGATGGCGAAACAAAGCCCGGAAAGCCAGGAGCGTATTACCGAAAAAGTAGAAATACTACG
TCAGGCGGTTGCGTTAAATATGTTAAGGGAAGAACTGAATCTCTCTCAGGCTGAACTTGCCTCGGCAATGGGGGTTAAGC
AGCCAACAATCGCAAAAATGGAGCAGGCAGATAACGATCCTCGTCTGTCGACGCTCAAGCGTTATGTCACGGCACTGGGT
GGTGAACTTAGCATTGATGTTAAGCTACCTACAGGTAAAAGAGTCGCGTTCTATCTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References