Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 5076679..5077333 Replicon chromosome
Accession NZ_CP090203
Organism Klebsiella pneumoniae strain JNKPN26

Toxin (Protein)


Gene name cptA Uniprot ID R4YHX2
Locus tag LXM99_RS24990 Protein ID WP_004144731.1
Coordinates 5076926..5077333 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID W8UQ37
Locus tag LXM99_RS24985 Protein ID WP_002916312.1
Coordinates 5076679..5076945 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LXM99_RS24960 (LXM99_24960) 5071835..5073268 - 1434 WP_002916322.1 6-phospho-beta-glucosidase BglA -
LXM99_RS24965 (LXM99_24965) 5073387..5074115 - 729 WP_002916321.1 MurR/RpiR family transcriptional regulator -
LXM99_RS24970 (LXM99_24970) 5074165..5074476 + 312 WP_002916319.1 N(4)-acetylcytidine aminohydrolase -
LXM99_RS24975 (LXM99_24975) 5074640..5075299 + 660 WP_002916317.1 hemolysin III family protein -
LXM99_RS24980 (LXM99_24980) 5075450..5076433 - 984 WP_002916313.1 tRNA-modifying protein YgfZ -
LXM99_RS24985 (LXM99_24985) 5076679..5076945 + 267 WP_002916312.1 FAD assembly factor SdhE Antitoxin
LXM99_RS24990 (LXM99_24990) 5076926..5077333 + 408 WP_004144731.1 protein YgfX Toxin
LXM99_RS24995 (LXM99_24995) 5077340..5077861 - 522 WP_002916308.1 flavodoxin FldB -
LXM99_RS25000 (LXM99_25000) 5077962..5078858 + 897 WP_004144729.1 site-specific tyrosine recombinase XerD -
LXM99_RS25005 (LXM99_25005) 5078881..5079594 + 714 WP_002916301.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
LXM99_RS25010 (LXM99_25010) 5079600..5081333 + 1734 WP_004151783.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 15936.69 Da        Isoelectric Point: 11.4778

>T230232 WP_004144731.1 NZ_CP090203:5076926-5077333 [Klebsiella pneumoniae]
VVLWQSDLRISWRAQWFSLLLHGVVAALVLLVPWPLSYTPIWLLLSLVVFDCVRSQRRIHARRGEIKLLTDSRLRWQNAE
WEILGTPWVINSGMLLRLRHVDTRRGQHLWLAADSMDAGEWRDLRRLVLQKPAQE

Download         Length: 408 bp

>T230232 NZ_CP124809:568059-568343 [Citrobacter freundii]
ATGACTTATGAACTGGAATTCGACCCGAGAGCCTTAAAAGAGTGGCATAAACTGGGTGATACGGTGAAGGCTCAGTTTAA
GAAGAAGTTGGCCGACGTGTTGTTGAACCCCAGAATTGACTCTGCCCGTTTAAACGGTCTTCCAGATTGCTATAAAATTA
AGCTTAAATCGTCCGGCTATCGTCTGGTGTACCAGGTTCGGGATGAGGTTGTGACCGTGTTTGTTGTCGCGGTCGGCAAG
CGACAACATTCGGCCGTTTATCTCGATGCAAACAAACGGCTTTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10480.98 Da        Isoelectric Point: 5.1116

>AT230232 WP_002916312.1 NZ_CP090203:5076679-5076945 [Klebsiella pneumoniae]
MDINNKARIHWACRRGMRELDISIMPFFEYEYDTLSDADKQLFIRLLENDDPDLFNWLMNHGKPADAELQRMVTLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT230232 NZ_CP124809:567827-568069 [Citrobacter freundii]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAATGAGGCTTATGCCGTGCTGGAAAAACTGAACATTACTCC
AACGGAAGCTGTGCGATTACTGTTTCAGTACGTCGCGGAAACGGGGCGCATGCCAGTGAAAACCGTCACCGTTAGCGACA
GCGAAGATACACTGATTCAGACAGTCCGGGAGAGGCTGGCCAGCCCGCAAAAAGGAATCAAGGTTAGTCTGGATGACTTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A378E4P6

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GY41

References