Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 4121792..4122417 Replicon chromosome
Accession NZ_CP090203
Organism Klebsiella pneumoniae strain JNKPN26

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag LXM99_RS20195 Protein ID WP_002882817.1
Coordinates 4121792..4122175 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag LXM99_RS20200 Protein ID WP_004150355.1
Coordinates 4122175..4122417 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LXM99_RS20180 (LXM99_20180) 4119158..4120060 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
LXM99_RS20185 (LXM99_20185) 4120057..4120692 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
LXM99_RS20190 (LXM99_20190) 4120689..4121618 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
LXM99_RS20195 (LXM99_20195) 4121792..4122175 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
LXM99_RS20200 (LXM99_20200) 4122175..4122417 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
LXM99_RS20205 (LXM99_20205) 4122622..4123539 + 918 WP_002882812.1 alpha/beta hydrolase -
LXM99_RS20210 (LXM99_20210) 4123553..4124494 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
LXM99_RS20215 (LXM99_20215) 4124539..4124976 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
LXM99_RS20220 (LXM99_20220) 4124973..4125833 - 861 WP_002882807.1 virulence factor BrkB family protein -
LXM99_RS20225 (LXM99_20225) 4125827..4126426 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T230228 WP_002882817.1 NZ_CP090203:c4122175-4121792 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T230228 NZ_CP124806:c26941-26525 [Klebsiella variicola]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT230228 WP_004150355.1 NZ_CP090203:c4122417-4122175 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT230228 NZ_CP124806:c27168-26938 [Klebsiella variicola]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References