Detailed information of TA system TA230201

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 3091074..3091858
• Replicon: chromosome
• Accession: NZ_CP090196
• Organism: Escherichia coli strain YL3

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: V0T0H9
• Locus tag: LXN07_RS15020
• Protein ID: WP_000613626.1
• Coordinates: 3091364..3091858 (+)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: L4JCW6
• Locus tag: LXN07_RS15015
• Protein ID: WP_001110447.1
• Coordinates: 3091074..3091367 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LXN07_RS15005 (3086224)	3086224..3087183	-	960	WP_000846343.1	23S rRNA pseudouridine(955/2504/2580) synthase RluC	-
LXN07_RS15010 (3087756)	3087756..3090941	+	3186	WP_001522867.1	ribonuclease E	-
LXN07_RS15015 (3091074)	3091074..3091367	+	294	WP_001110447.1	DUF1778 domain-containing protein	Antitoxin
LXN07_RS15020 (3091364)	3091364..3091858	+	495	WP_000613626.1	GNAT family N-acetyltransferase	Toxin
LXN07_RS15025 (3091953)	3091953..3092906	-	954	WP_001212768.1	flagellar hook-associated protein FlgL	-
LXN07_RS15030 (3092918)	3092918..3094561	-	1644	WP_001522865.1	flagellar hook-associated protein FlgK	-
LXN07_RS15035 (3094627)	3094627..3095568	-	942	WP_001522863.1	flagellar assembly peptidoglycan hydrolase FlgJ	-
LXN07_RS15040 (3095568)	3095568..3096665	-	1098	WP_000589326.1	flagellar basal body P-ring protein FlgI	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (81-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-88)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 18084.00 Da
• Isoelectric Point: 8.2617

>T230201 WP_000613626.1 NZ_CP090196:3091364-3091858 [Escherichia coli]
MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIGRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Nucleotide

• Length: 495 bp

>T230201 NZ_CP124754:438309-438584 [Serratia sp. K-E0102]
ATGCGTATCGAATGGGATAATGCAGCGCTACGGGACAGGGAACGTATTTTTGAGTTTCTTTATATTTTCAATCCACAGGC
GGCAGTGCAGGCTGATAACGAGATAGATAAAGCGGTAAAGCGCCTGCTGGATCATCCTGAGTTAGGTAAGCCTTGGTATG
GGAAGGCACGTAAGCTGCTCATTAACAAGGCCTCCCTGTTGCTGGTGTATATCATCGTCGATGATGTTATTAAGGTTTTC
TCGATTGCCCACCAGCGTGAGAAATTTCCAAATTAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11109.69 Da
• Isoelectric Point: 10.5423

>AT230201 WP_001110447.1 NZ_CP090196:3091074-3091367 [Escherichia coli]
MPAANNIPTRRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Nucleotide

• Length: 294 bp

>AT230201 NZ_CP124754:438013-438300 [Serratia sp. K-E0102]
ATGGAAAGTCGCATCCAGTTTCGCATCGAAAATGAAACCAAGCGTTTGGCGCAAAAGGCCGCCGAGGCTAAAGGAACGAC
ATTAAGTGAAGCCTGTCGCCGCTTGGCGGAGCAAATGGCTGATGAGCAACGGGCCGCAGAGGAACACGAAAACTGGTTGA
AAGATAAGGTAGATGCAGCTTTTTCCCGTCTGCATGAGGGTAATGCTGTTTACCTTAGCCAACAAGAAGTAGAAGAAAGC
ATGGATGCCTTTAAGGCAAAAATTAGAGCGAAATACGACCGAAAATAG

Structures

Toxin

• AlphaFold DB: V0T0H9

Antitoxin

• AlphaFold DB: L4JCW6