Detailed information of TA system TA230200

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2569464..2570102
• Replicon: chromosome
• Accession: NZ_CP090196
• Organism: Escherichia coli strain YL3

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A0D8WF76
• Locus tag: LXN07_RS12335
• Protein ID: WP_001306887.1
• Coordinates: 2569464..2569640 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: LXN07_RS12340
• Protein ID: WP_001270286.1
• Coordinates: 2569686..2570102 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LXN07_RS12310 (2564520)	2564520..2564978	+	459	WP_000526135.1	IS200/IS605-like element IS200C family transposase	-
LXN07_RS12315 (2565086)	2565086..2566258	-	1173	WP_001523225.1	BenE family transporter YdcO	-
LXN07_RS12320 (2566350)	2566350..2566886	+	537	WP_020233877.1	DNA-binding transcriptional regulator SutR	-
LXN07_RS12325 (2566959)	2566959..2568920	+	1962	WP_024166512.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
LXN07_RS12330 (2569012)	2569012..2569242	-	231	WP_000494241.1	YncJ family protein	-
LXN07_RS12335 (2569464)	2569464..2569640	+	177	WP_001306887.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
LXN07_RS12340 (2569686)	2569686..2570102	+	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
LXN07_RS12345 (2570181)	2570181..2571587	+	1407	WP_001523229.1	PLP-dependent aminotransferase family protein	-
LXN07_RS12350 (2571832)	2571832..2572977	+	1146	WP_020233879.1	ABC transporter substrate-binding protein	-
LXN07_RS12355 (2572995)	2572995..2574008	+	1014	WP_001523231.1	ABC transporter ATP-binding protein	-
LXN07_RS12360 (2574009)	2574009..2574950	+	942	WP_001251315.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2564520..2564978	458

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6768.88 Da
• Isoelectric Point: 11.5336

>T230200 WP_001306887.1 NZ_CP090196:2569464-2569640 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFRGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T230200 NZ_CP124754:57595-57870 [Serratia sp. K-E0102]
ATGGCGTTAAAATGGACGAGTAAGGCGCTATCGGATTTGGCGCGTTTGTTTGAGTTTCTTGCCTTGGCTAACCGTACTGC
GGCAGCGAATTCGGTGAAGGCCTTGGTCGCCGCGCCTAAAGCGTTGCTTGATAATCCTCGCCTGGGTGAAAGACTGGACG
AGTTTTTACCGCAGGAAGTGAGGCGTATTCTGGTCGGCCGTTATGAGATACGTTACCAGGTGCAGCCGGCAACGATTTAT
GTTCTGCGTATTTGGCATCCCCGCGAAGAGCGATAG

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT230200 WP_001270286.1 NZ_CP090196:2569686-2570102 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT230200 NZ_CP124754:57335-57595 [Serratia sp. K-E0102]
ATGAATACGAAAGTCATCACCGCTCATGTGCCGCTGCCTTTGGCTGAAAAGGTGGATGAAATGGCCGCCAAACTTGAACG
CTCACGGGGATGGATAGTGAAGCAAGCCCTCACCGCCTGGCTCGAGCAAGAGGAAGAGCGCCGTCGATTAACGCTGGCGG
CGCTGGCCGATGTCGAACAGCAAAACGTTATCGATCATGAATCGGTAGAAGCCTGGGCTGCCAGCCTGGACAGTGACGAT
CCGCTGCCGGTACCGCGCTGA

Structures

Toxin

• AlphaFold DB: A0A0D8WF76