Detailed information of TA system TA230154

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1250311..1251228
• Replicon: chromosome
• Accession: NZ_CP090150
• Organism: Bacillus velezensis strain BR-01

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: I2HQ15
• Locus tag: LXH20_RS06530
• Protein ID: WP_007407256.1
• Coordinates: 1250482..1251228 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2HQ14
• Locus tag: LXH20_RS06525
• Protein ID: WP_003154807.1
• Coordinates: 1250311..1250481 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LXH20_RS06485 (LXH20_06485)	1245544..1247166	+	1623	WP_022553476.1	pyocin knob domain-containing protein	-
LXH20_RS06490 (LXH20_06490)	1247179..1247550	+	372	WP_022553475.1	XkdW family protein	-
LXH20_RS06495 (LXH20_06495)	1247555..1247752	+	198	WP_007610833.1	XkdX family protein	-
LXH20_RS06500 (LXH20_06500)	1247809..1248570	+	762	WP_022553474.1	hypothetical protein	-
LXH20_RS06505 (LXH20_06505)	1248622..1248885	+	264	WP_022553473.1	hemolysin XhlA family protein	-
LXH20_RS06510 (LXH20_06510)	1248899..1249162	+	264	WP_003154813.1	phage holin	-
LXH20_RS06515 (LXH20_06515)	1249176..1250054	+	879	WP_007407257.1	N-acetylmuramoyl-L-alanine amidase	-
LXH20_RS06520 (LXH20_06520)	1250089..1250214	-	126	WP_003154809.1	hypothetical protein	-
LXH20_RS06525 (LXH20_06525)	1250311..1250481	-	171	WP_003154807.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
LXH20_RS06530 (LXH20_06530)	1250482..1251228	-	747	WP_007407256.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
LXH20_RS06535 (LXH20_06535)	1251333..1252331	-	999	WP_003154805.1	inorganic phosphate transporter	-
LXH20_RS06540 (LXH20_06540)	1252344..1252961	-	618	WP_003154804.1	DUF47 domain-containing protein	-
LXH20_RS06545 (LXH20_06545)	1253247..1254563	-	1317	WP_007610842.1	amino acid permease	-
LXH20_RS06550 (LXH20_06550)	1254885..1255835	+	951	WP_022553472.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29077.57 Da
• Isoelectric Point: 4.7755

>T230154 WP_007407256.1 NZ_CP090150:c1251228-1250482 [Bacillus velezensis]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T230154 NZ_CP124737:1974854-1975117 [Limosilactobacillus fermentum]
ATGAGTTACGCGATCAAACTCAAGCGGAGCGCTAATAAAGATCTCAAGAAAATTAAGGGGTCATATTTAGAAAAGAGCTT
CTTGCAAATAATTGAGCAGCTCCGTAAAGACCCCTTGGCACCCAACCAAGGCTTTGAAAAATTAGTACTGCCAATTAAGG
GCTTTTACTCCCGGCGGATTAACATTCAGCACCGACTCGTCTATAAGGTTGATCAAGACACGCAAACTGTGATCATCTAT
TCTGCGTGGTCACATGACGAATAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.74 Da
• Isoelectric Point: 10.9273

>AT230154 WP_003154807.1 NZ_CP090150:c1250481-1250311 [Bacillus velezensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT230154 NZ_CP124737:1974603-1974857 [Limosilactobacillus fermentum]
ATGGACCAAATTGTAACCCCAACGCAAGCACGGAAAGATTTATTCAAAATAATCAAAAGGATCAATGAAAATAATGAGCC
GGTTGTGATTAAGCCGACCAGGGCGGATGAAAAGAGCGTTGTCGTCATTGGTGAAGATGACTGGAAAGCAATCCAAGAAA
CGTTATTTCTGGTTAATCACGGTGTTGACAAGCAAATTAAAGAACGGGAAAACGAGCCGCTCGACGACTTTGATGATGTT
TGGGGTCAACTATGA

Structures

Toxin

• AlphaFold DB: I2HQ15

Antitoxin

• AlphaFold DB: I2HQ14