Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | mazEF/MazF(toxin) |
| Location | 482192..482829 | Replicon | chromosome |
| Accession | NZ_CP090150 | ||
| Organism | Bacillus velezensis strain BR-01 | ||
Toxin (Protein)
| Gene name | mazF | Uniprot ID | G4NU33 |
| Locus tag | LXH20_RS02455 | Protein ID | WP_003156187.1 |
| Coordinates | 482479..482829 (+) | Length | 117 a.a. |
Antitoxin (Protein)
| Gene name | mazE | Uniprot ID | I2HMS5 |
| Locus tag | LXH20_RS02450 | Protein ID | WP_003156188.1 |
| Coordinates | 482192..482473 (+) | Length | 94 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| LXH20_RS02430 (LXH20_02430) | 478557..479156 | - | 600 | WP_003156193.1 | rhomboid family intramembrane serine protease | - |
| LXH20_RS02435 (LXH20_02435) | 479249..479614 | + | 366 | WP_022552632.1 | holo-ACP synthase | - |
| LXH20_RS02440 (LXH20_02440) | 479779..480786 | + | 1008 | WP_007410230.1 | outer membrane lipoprotein carrier protein LolA | - |
| LXH20_RS02445 (LXH20_02445) | 480903..482072 | + | 1170 | WP_022552633.1 | alanine racemase | - |
| LXH20_RS02450 (LXH20_02450) | 482192..482473 | + | 282 | WP_003156188.1 | type II toxin-antitoxin system antitoxin EndoAI | Antitoxin |
| LXH20_RS02455 (LXH20_02455) | 482479..482829 | + | 351 | WP_003156187.1 | type II toxin-antitoxin system endoribonuclease NdoA | Toxin |
| LXH20_RS02460 (LXH20_02460) | 482947..483768 | + | 822 | WP_003156182.1 | STAS domain-containing protein | - |
| LXH20_RS02465 (LXH20_02465) | 483773..484138 | + | 366 | WP_007609584.1 | RsbT antagonist protein RsbS | - |
| LXH20_RS02470 (LXH20_02470) | 484141..484542 | + | 402 | WP_003156178.1 | anti-sigma regulatory factor | - |
| LXH20_RS02475 (LXH20_02475) | 484554..485561 | + | 1008 | WP_007609589.1 | PP2C family protein-serine/threonine phosphatase | - |
| LXH20_RS02480 (LXH20_02480) | 485625..485954 | + | 330 | WP_003156176.1 | anti-sigma factor antagonist RsbV | - |
| LXH20_RS02485 (LXH20_02485) | 485951..486433 | + | 483 | WP_007609591.1 | anti-sigma B factor RsbW | - |
| LXH20_RS02490 (LXH20_02490) | 486399..487187 | + | 789 | WP_003156171.1 | RNA polymerase sigma factor SigB | - |
| LXH20_RS02495 (LXH20_02495) | 487187..487789 | + | 603 | WP_007410234.1 | PP2C family serine/threonine-protein phosphatase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 117 a.a. Molecular weight: 12977.98 Da Isoelectric Point: 4.8781
>T230153 WP_003156187.1 NZ_CP090150:482479-482829 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF
Download Length: 351 bp
>T230153 NZ_CP124733:2668535-2668804 [Agrobacterium larrymoorei]
TTGATCTGGGAAATTGAATTTCGAGAGTCTGCGCGCAAGCAATTGAAGAAGCTTGGCAGACAGGATGCAACGCGGATCAT
TGCATTTTTGAGTGATCGGATTTCCGCAGACACAAATCCAAGACGAACCGGTCAGGCACTTCAAGGCTCAGAGCTCGGCA
ACTTCTGGCGTTATCGCGTCGGTGACTATCGTATTATATGCGACATTCAGGATCAAAAACTTGTCATTATCGTCATCGAA
ATCGGCCACCGCCGGGAAGTCTATCGTTAA
TTGATCTGGGAAATTGAATTTCGAGAGTCTGCGCGCAAGCAATTGAAGAAGCTTGGCAGACAGGATGCAACGCGGATCAT
TGCATTTTTGAGTGATCGGATTTCCGCAGACACAAATCCAAGACGAACCGGTCAGGCACTTCAAGGCTCAGAGCTCGGCA
ACTTCTGGCGTTATCGCGTCGGTGACTATCGTATTATATGCGACATTCAGGATCAAAAACTTGTCATTATCGTCATCGAA
ATCGGCCACCGCCGGGAAGTCTATCGTTAA
Antitoxin
Download Length: 94 a.a. Molecular weight: 10562.92 Da Isoelectric Point: 6.4706
>AT230153 WP_003156188.1 NZ_CP090150:482192-482473 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG
Download Length: 282 bp
>AT230153 NZ_CP124733:2668318-2668551 [Agrobacterium larrymoorei]
ATGAGCAAACAAACCGCAATCCGTCTGCCGGATGAGACCTATGAGCGCCTGAAGGCGCTTTCCGAGCGCACGGGCCGCAC
CTCCGCTTATTACATCCGCGAAGCCATCGAAAAGCACATCGAGGATATGGAGGATCTGTATCTGGCGGAAGAGGCGACGC
GGCGACACATTGCAAATGTTGAGCGCACCTATAGTCTTGAAGAAGTGATGCGCGATCTTGATCTGGGAAATTGA
ATGAGCAAACAAACCGCAATCCGTCTGCCGGATGAGACCTATGAGCGCCTGAAGGCGCTTTCCGAGCGCACGGGCCGCAC
CTCCGCTTATTACATCCGCGAAGCCATCGAAAAGCACATCGAGGATATGGAGGATCTGTATCTGGCGGAAGAGGCGACGC
GGCGACACATTGCAAATGTTGAGCGCACCTATAGTCTTGAAGAAGTGATGCGCGATCTTGATCTGGGAAATTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|